Study Objectives:

Methylene blue (MB) is an antioxidant that ameliorates ischemia-reperfusion injury in several tissues. We analyzed the effects of MB as an inhibitor of torsion–detorsion injury in rat ovaries.

Methods

Rats were randomly divided into 5 groups. Group 1 was the sham group, in which only laparotomy was performed. Group 2 was the torsion group, with 3 hours of ischemia. Group 3 was the torsion + MB group, with 3 hours of ischemia after MB administration. Group 4 was the torsion–detorsion group, with 3 hours of ischemia and reperfusion. Finally, group 5 was the torsion–detorsion + MB group, with 3 hours of ischemia and MB administration before detorsion/reperfusion. Ovary injuries were histopathologically scored. Malondialdehyde (MDA) and total protein levels in ovarian tissues were determined, and long pentraxin-3 (PTX3) levels were measured in ovarian tissue using an enzyme-linked immunosorbent assay.

Results

In comparing group 4 with group 5 and group 2 with 3, histopathological parameters reflecting injury were significantly increased in groups 4 and 2. Group 3 generated increased MDA levels when compared with group 2 (P < .05). However, there was no significant difference between groups 2 and 3 in terms of plasma PTX3 levels. MDA and PTX3 levels decreased in group 5 in comparison with group 4 for MDA (P < .000) and PTX3 levels (P < .01).

Conclusions

MB alleviated ischemia-reperfusion ovary injury in our experimental model.

中文翻译：

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亚甲蓝对大鼠模型卵巢扭转扭曲损伤的影响。

研究目标：

亚甲蓝（MB）是一种抗氧化剂，可改善多个组织的缺血再灌注损伤。我们分析了MB作为大鼠卵巢扭转扭曲损伤抑制剂的作用。

方法

将大鼠随机分为5组。第一组是假手术组，仅进行剖腹手术。第2组为扭转组，缺血3小时。第3组为扭转+ MB组，MB给药后缺血3小时。第4组为扭转-扭转组，进行3小时的缺血和再灌注。最后，第5组是扭转-扭曲+ MB组，在扭曲/再灌注之前进行3小时的局部缺血和MB治疗。对卵巢损伤进行组织病理学评分。测定了卵巢组织中的丙二醛（MDA）和总蛋白水平，并使用酶联免疫吸附测定法测量了卵巢组织中的长pentraxin-3（PTX3）水平。

结果

比较第4组和第5组，第2组和第3组，反映损伤的组织病理学参数在第4和第2组显着增加。与第2组相比，第3组产生的MDA水平升高（P  <.05）。然而，就血浆PTX3水平而言，第2组和第3组之间没有显着差异。与MDA的第4组相比，第5组的MDA和PTX3水平降低（P  <.000）和PTX3的水平（P  <.01）。

结论

MB在我们的实验模型中减轻了缺血-再灌注卵巢损伤。