Stripe rust is a continuous threat to wheat crop all over the world. It causes considerable yield losses in wheat crop every year. Continuous deployment of adult plant resistance (APR) genes in newly developing wheat cultivars is the most judicious strategy to combat this disease. Herein, we dissected the genetics underpinning stripe rust resistance in Pakistani wheat germplasm. An association panel of 94 spring wheat genotypes was phenotyped for two years to score the infestation of stripe rust on each accession and was scanned with 203 polymorphic SSRs. Based on D´ measure, linkage disequilibrium (LD) exhibited between loci distant up to 45 cM. Marker-trait associations (MTAs) were determined using mixed linear model (MLM). Total 31 quantitative trait loci (QTLs) were observed on all 21 wheat chromosomes. Twelve QTLs were newly discovered as well as 19 QTLs and 35 previously reported Yr genes were validated in Pakistani wheat germplasm. The major QTLs were QYr.uaf.2AL and QYr.uaf.3BS (PVE, 11.9%). Dissection of genes from the newly observed QTLs can provide new APR genes to improve genetic resources for APR resistance in wheat crop.

条锈病一直是世界范围内对小麦作物的持续威胁。每年造成小麦作物的大量减产。在新兴的小麦品种中持续部署成年植物抗性（APR）基因是与这种疾病抗争的最明智的策略。在这里，我们解剖了巴基斯坦小麦种质条纹抗锈性的遗传学基础。对94个春小麦基因型的关联小组进行了两年的表型分析，以对每个种质上条锈病的侵害进行评分，并用203个多态性SSR进行了扫描。基于D´测量时，距离不超过45 cM的基因座之间表现出连锁不平衡（LD）。使用混合线性模型（MLM）确定标记-性状关联（MTA）。在所有21条小麦染色体上共观察到31个数量性状基因座（QTL）。新发现了12个QTL，以及19个QTL，并在巴基斯坦小麦种质中验证了35个先前报道的Yr基因。主要的QTL是QYr.uaf.2AL和QYr.uaf.3BS（PVE，11.9％）。从新观察到的QTLs中分离基因可以提供新的APR基因，以改善小麦作物抗APR的遗传资源。