Journal of Integrative Agriculture ( IF 4.6 ) Pub Date : 2020-06-24 , DOI: 10.1016/s2095-3119(19)62806-6 Ya-qin WANG , Yu SONG , Meng-ji CAO , Qi CHENG , Jian-xiang WU , Tao HU
Emaraviruses are multipartite negative-sense single-stranded viruses that usually infect flowering plants. Using unbiased next-generation sequencing (NGS) approach, a novel virus from lilac leaves with yellow mottle symptom was identified. Five viral genomic RNA segments were detected and completely sequenced. The molecular characteristics of the viral RNAs and proteins resemble those of members of the genus Emaravirus. The virus was found to be mechanically transmissible to Nicotiana benthamiana. Sequence alignment and phylogenetic analyses of the virus support the proposal of creating a new species in the genus Emaravirus for which the name lilac chlorotic ringspot-associated virus (LiCRaV) is proposed.
中文翻译:
通过下一代测序鉴定感染丁香的新型Emaravirus
Emaraviruses病毒是多部分负义单链病毒,通常会感染开花植物。使用无偏倚的下一代测序(NGS)方法,从淡紫色斑点中发现带有黄色斑点症状的新型病毒。检测到五个病毒基因组RNA片段并进行了完整测序。病毒RNA和蛋白质的分子特征类似于埃马拉病毒属的成员。发现该病毒可机械传播给本特烟草。该病毒的序列比对和系统发育分析支持在埃玛拉病毒属中创建一个新物种的提议,该物种的名称为淡紫色的与环绿环相关的病毒(LiCRaV)。