Previous studies on mammals showed that peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) played a prominent role in regulating muscle fiber type transition and composition. However, the role of PGC-1α in chicken muscle has seldom been explored. To investigate the effect of PGC-1α on chicken skeletal muscles in this study, the PGC-1α gene was overexpressed or silenced in chicken primary myoblasts by using lentivirus, and then the effects of the PGC-1α gene overexpression and knockdown on the mRNA expression profile of genes related to myofiber type specificity were examined during fiber formation. The results showed that overexpression of PGC-1α from proliferation to differentiation was accompanied by the up-regulated expression of Pax7, MyoD, and CnAα, which was significantly (P<0.01) increased after one day of transfection (1I). The enhancement of MyoG, MEF2c, and MyHC SM expression lagged, which was improved significantly (P<0.01) after four days of transfection (1I3D). Overexpression of PGC-1α decreased (P<0.01) the MyHC FWM expression after four days of transfection (1I3D), and it had no significant impact (P>0.05) on the expression of CnB1, NFATc3, and MyHC FRM during myofiber formation. The effective silence (P<0.01) of PGC-1α by lentivirus mediating short hairpin RNA (shRNA) was detected after four days of transfection (1I3D) in cultures, and the lack of its function in chicken primary myoblasts significantly (P<0.01) down-regulated the expression of Pax7, MyoD, CnAα, MyoG, MEF2c, and MyHC SM, significantly (P<0.01) up-regulated the expression of MyHC FWM, and had no significant impact (P>0.05) on the expression of CnB1, NFATc3, and MyHC FRM. These results indicated that the role of PGC-1α in regulating the fiber type specificity of chicken skeletal muscles might be similar to that in mammals, which interplayed with key genes related to myocyte differentiation and calcineurin signaling pathway.

先前对哺乳动物的研究表明，过氧化物酶体增殖物激活的受体γcoactivator-1α（PGC-1α）在调节肌纤维类型的转变和组成中起着重要作用。然而，很少研究PGC-1α在鸡肌肉中的作用。为了研究PGC-1α对鸡骨骼肌的影响，本研究使用慢病毒在鸡原代成肌细胞中过表达或沉默了PGC-1α基因，然后研究了PGC-1α基因的过表达和敲低对mRNA表达的影响。在纤维形成过程中检查了与肌纤维类型特异性相关的基因的概况。结果表明，PGC-1α从增殖到分化的过度表达伴随着pGC-1α的上调表达。转染一天（1I）后，Pax7，MyoD和CnAα显着增加（P < 0.01）。转染四天（1I3D）后，MyoG，MEF2c和MyHC SM表达的增强滞后，而显着改善（P < 0.01）。转染4天（1I3D）后，PGC-1α的过表达降低（P < 0.01）MyHC FWM表达，并且在肌纤维形成过程中对CnB1，NFATc3和MyHC FRM的表达无显着影响（P > 0.05）。的有效静音（P < 0.01）转染四天后（1I3D），在慢病毒介导的短发夹RNA（shRNA）中检测到PGC-1α，并且在鸡原代成肌细胞中缺乏PGC-1α的功能显着（P < 0.01）下调了Pax7，MyoD的表达，CnAα，MyoG，MEF2c和MyHC SM显着（P < 0.01）上调了MyHC FWM的表达，对CnB1，NFATc3和MyHC FRM的表达没有显着影响（P > 0.05）。这些结果表明PGC-1α的作用 在调节鸡骨骼肌纤维类型特异性方面的作用可能与哺乳动物相似，它们与与肌细胞分化和钙调神经磷酸酶信号通路有关的关键基因相互作用。