Two novel Co(II) fenamato complexes containing bathocuproine (bcp), namely [Co(bcp)(flu)₂] (1) and [Co(bcp)(nif)₂] (2) (flu = flufenamato, nif = niflumato) were synthesized and characterized by elemental analysis, single-crystal X-ray structure analysis as well as absorption and fluorescence spectroscopy. Investigation of their molecular structure revealed that both complexes are isostructural and form analogous complex molecules, with a Co(II) atom hexacoordinated by two nitrogen atoms of bcp and four oxygen atoms of two chelate bonded flu (1) and nif (2) ligands in a distorted octahedral arrangement. Surprisingly, the results of cytotoxicity experiments on four cancer cell lines (HeLa, HT-29, PC-3 and MCF-7) have revealed that despite similar structure of the complexes, the nif complex exhibits significantly higher activity, being the most effective against the PC-3 cell line (IC50 (MTT) = 6.11 ± 1.95 μM). Further studies performed on PC-3 cell line have shown that the mechanism of the cytotoxic action of nif complex (2) might involve activation of autophagic processes and apoptosis which was not detected in case of its flu analogue (1).

两种新的含浴铜蛋白（bcp）的Co（II）fenamato络合物，即[Co（bcp）（flu）₂ ]（1）和[Co（bcp）（nif）₂ ]（2）（flu  = flufenamato，nif  = niflumato通过元素分析，单晶X射线结构分析以及吸收和荧光光谱进行合成和表征。对它们的分子结构的研究表明，这两种配合物都是同构的，并形成类似的配合物分子，Co（II）原子与bcp的两个氮原子六配位和四个螯合键的flu（1）和nif（2）配体的四个氧原子以扭曲的八面体排列。出乎意料的是，对四种癌细胞系（HeLa，HT-29，PC-3和MCF-7）的细胞毒性实验结果表明，尽管复合物的结构相似，但nif复合物仍显示出更高的活性，是最有效的抗PC-3细胞系（IC50（MTT）= 6.11±1.95μM）。对PC-3细胞系进行的进一步研究表明，nif复合物（2）的细胞毒性作用机制可能涉及自噬过程的激活和细胞凋亡，而在流感的情况下则未检测到类似物（1）。