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Kinetic and thermodynamic studies of cinnamycin specific-adsorption on PE-Included-Membranes using surface plasmon resonance.
Journal of Biotechnology ( IF 4.1 ) Pub Date : 2020-06-25 , DOI: 10.1016/j.jbiotec.2020.06.020
Sang-Ryong Lee 1 , Yeseul Park 2 , Jin-Won Park 2
Affiliation  

The binding of the cinnamycin on the biomimetic membrane was studied with respect to time using the surface plasmon resonance(SPR). The membrane was composed of the inner layer tethered on the gold surface and the outer layer formed on the inner layer, which was at the desired ratio of dioleoylphosphatidylethanolamine(DOPE) to dioleoylphosphatidyl- choline(DOPC). On the bilayer, the cinnamycin solution was injected and showed different behavior of the binding with respect to time up on its concentration. For kinetic analysis, the behavior was converted to the coverage fraction with respect to time, which was ratio to the saturated response of 5 μM cinnamycin solution. The fraction change with respect to time was function of the available-site, which was eventually the subtraction of the fraction from one. With the fitting of the first order of the available site, the rate constant was acquired into 6∼7 × 10−3 s-1. Furthermore, the reciprocals of the fraction and the concentration were fitted with the Langmuir adsorption isotherm. From the fitting, the equilibrium constant was between 1 × 107 and 5 × 107 M-1.



中文翻译:

使用表面等离振子共振动力学和热力学研究肉桂霉素在PE包括的膜上的特异性吸附。

利用表面等离振子共振(SPR)研究了肉桂霉素在仿生膜上的结合作用。该膜由束缚在金表面上的内层和形成在内层上的外层组成,该外层的比例为二油酰基磷脂酰乙醇胺(DOPE)与二油酰基磷脂酰胆碱(DOPC)的理想比例。在双层上注射肉桂霉素溶液,并显示其结合时间随浓度的升高而变化。对于动力学分析,将行为转换为相对于时间的覆盖率,该覆盖率与5μM肉桂霉素溶液的饱和响应之比。分数随时间的变化是可用位点的函数,最终是分数从一处减去。×10 -3 s -1。此外,馏分和浓度的倒数与Langmuir吸附等温线拟合。根据拟合,平衡常数在1与×10 7和5×10 7 M -1

更新日期:2020-06-28
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