While many studies have been conducted on drug-inducing alterations in the aquatic environment, little is known about their interaction with climate change, such as rising temperatures. To increase knowledge on this topic, Mytilus galloprovincialis mussels were exposed to two different temperatures 17 ± 1 °C (control) and 21 ± 1 °C in the absence and presence of salicylic acid (SA) (4 mg/L) for 28 days. Salicylic acid in the water and tissues was measured and its impact reported through biomarker responses including: energy metabolism (electron transport system (ETS) activity, glycogen (GLY), protein (PROT) and lipids (LIP) contents), oxidative stress markers (activity of the enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)), glutathione balance between the reduced and the oxidized forms (GSH/GSSG), and damage to membrane lipids (lipid peroxidation - LPO). The mussels responded differently if the stresses imposed were single or combined, with greater impacts when both stressors were acting together. Contaminated mussels exposed to high temperatures were unable to increase their metabolic capacity to restore their defence mechanisms, reducing the expenditure of LIP. In the presence of SA and increased temperature antioxidant defences respond differently, with higher SOD levels and inhibition of CAT. The present study highlights not only the negative impact of warming and SA, but especially how temperature increase will promote the impact of SA in M. galloprovincialis, which under predicted climate change scenarios may greatly impair population maintenance and ecosystem biodiversity.

尽管已经对水生环境中的药物诱发变化进行了许多研究，但对它们与气候变化（例如温度升高）的相互作用了解甚少。为了增加对此主题的了解，Mytilus galloprovincialis在不存在和存在水杨酸（SA）（4 mg / L）的情况下，贻贝暴露于17±1°C（对照）和21±1°C的两个不同温度下28天。测量了水和组织中的水杨酸，并通过生物标记响应报告了其影响，包括生物代谢，能量代谢（电子传输系统（ETS）活性，糖原（GLY），蛋白质（PROT）和脂质（LIP）含量），氧化应激标记（超氧化物歧化酶（SOD），过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性，还原型和氧化型之间的谷胱甘肽平衡（GSH / GSSG）以及对膜脂质的损害（脂质过氧化-LPO）。如果施加的压力是单一的还是组合的，贻贝的反应会有所不同，当两个压力源共同作用时，贻贝的影响会更大。受污染的贻贝在高温下无法提高其代谢能力以恢复防御机制，从而减少了LIP的支出。在存在SA和温度升高的情况下，抗氧化剂的防御反应有所不同，SOD含量较高且对CAT的抑制作用较高。本研究不仅强调了变暖和水杨酸的负面影响，而且还特别强调了温度升高将如何促进水杨酸和水杨酸的影响。M. galloprovincialis，在预测的气候变化情景下可能极大地损害人口维持和生态系统生物多样性。