The presence of tetracycline is ubiquitous and has adverse effects on aquatic systems. A hydroponic experiment was conducted to investigate the ecological sensitivity of Hydrocharis dubia (Bl.) Backer and Trapa bispinosa Roxb. Exposed to different concentrations of tetracycline (0, 0.1, 1, 10, 30 and 50 mg/L) for one day (1D) and 14 days (14D). The results showed that after 1D of tetracycline exposure, the physiological indices of H. dubia had no remarkable change except for proline which was significantly stimulated under 0.1 mg/L tetracycline. For T. bispinosa, guaiacol peroxidase (POD), polyphenol oxidase (PPO) and ascorbate peroxidase (APX) activity and protein and proline content were notably promoted under different concentrations of tetracycline, but PPO activity was significantly decreased in 50 mg/L. After 14D, tetracycline caused no harm to the growth and protein content of H. dubia, but negatively influenced lipid peroxidation product and chlorophyll content in H. dubia under high tetracycline concentrations. Superoxide dismutase (SOD) and POD activity of H. dubia significantly increased at high tetracycline concentrations, while catalase (CAT) and PPO activity significantly decreased. APX activity in H. dubia increased with tetracycline concentrations at low tetracycline concentrations. For T. bispinosa, high concentrations of tetracycline application significantly inhibited its growth and the content of protein and chlorophyll. SOD, POD, CAT, and PPO activity of T. bispinosa were induced under different concentrations of tetracycline and no lipid peroxidation was observed. APX activity in T. bispinosa was significantly inhibited at high tetracycline concentrations. The results suggest that tetracycline can cause oxidative damage in H. dubia but harm the metabolism process of T. bispinosa without inducing oxidative damage. Overall, the sensitivity of T. bispinosa exposed to tetracycline exposure is higher than that of H. dubia.

四环素的存在无处不在，对水生系统有不利影响。进行了一项水培实验，以研究杜氏烟草（Hydrocharis dubia（Bl。）Backer和Trapa bispinosa Roxb ）的生态敏感性。一天（1D）和14天（14D）暴露于不同浓度的四环素（0、0.1、1、10、30和50 mg / L）。结果表明，四环素暴露1D后，除0.1 mg / L四环素能显着刺激脯氨酸外，H。dubia的生理指标无明显变化。对于T. bispinosa在不同浓度的四环素下，愈创木酚过氧化物酶（POD），多酚氧化酶（PPO）和抗坏血酸过氧化物酶（APX）的活性以及蛋白质和脯氨酸含量显着提高，但在50 mg / L时PPO活性显着降低。14D后，四环素没有造成伤害的生长和蛋白质含量H.鳖，但脂质过氧化产物及叶绿素含量的负面影响H.鳖在高浓度的四环素。在高四环素浓度下，H。dubia的超氧化物歧化酶（SOD）和POD活性显着增加，而过氧化氢酶（CAT）和PPO活性则显着下降。H. dubia中的APX活动在低四环素浓度下，四环素浓度增加。对于双锥山毛榉，高浓度的四环素施用显着抑制了其生长以及蛋白质和叶绿素含量。在不同浓度的四环素下诱导了双尖顶梭菌的SOD，POD，CAT和PPO活性，未观察到脂质过氧化。在高四环素浓度下，T。bispinosa中的APX活性受到显着抑制。结果表明，四环素可以在H. dubia中引起氧化损伤，但在不引起氧化损伤的情况下损害bispinosa的代谢过程。总体而言，T。bispinosa的敏感性暴露于四环素的暴露高于假链藻。