This work was aimed to assess the contribution of classical nitrogen removal pathways in two deep constructed wetlands CW1 and CW2 located at Jaipur, India. Nitrogen mass balance revealed that 44.87% and 43.77% losses of T-N in CW1 and CW2 were unaccounted for. To elucidate these significant losses, the study was extended to assess the occurrence and contribution of a novel pathway (ANAMMOX) in overall nitrogen removal. The ratio of NH⁴⁺-N (removed) & NO₃⁻-N (produced) in CW1 & CW2 indicated that ANAMMOX could be one of the key pathways for nitrogen removal in the CWs besides nitrification-denitrification in microbial films. The molecular analysis confirmed bands of ANAMMOX bacteria developed intrinsically. The study revealed that deep wetlands can offer a feasible option for the sustenance of ANAMMOX bacteria and may help develop design parameters for CWs for achieving higher T-N removal with similar surface area as that of conventional wetlands.

这项工作旨在评估位于印度斋浦尔的两个深层人工湿地CW1和CW2中经典脱氮途径的贡献。氮的质量平衡表明，CW1和CW2中TN的损失为44.87％和43.77％。为了阐明这些重大损失，该研究已扩展到评估新型脱氮途径（ANAMMOX）的发生和贡献。NH的比值⁴⁺ -N（除去）NO ₃ ^-CW1和CW2中的-N（产生）表明，除了微生物膜中的硝化-反硝化作用外，ANAMMOX可能是CW中脱氮的关键途径之一。分子分析证实了ANAMMOX细菌的条带是固有发育的。研究表明，深部湿地可以为维持ANAMMOX细菌提供可行的选择，并可能有助于为连续水体开发设计参数，以实现更高的TN去除率，且表面积与常规湿地相近。