The innovative CRISPR-Cas based genome editing technology provides some functionality and advantages such as the high efficiency and specificity as well as ease of handling. Both aspects of the CRISPR-Cas9 system including genetic engineering and gene regulation are advantageously applicable to the construction of microbial cell factories. As one of the most extensively used cell factories, E. coli has been engineered to produce various high value-added chemical compounds such as pharmaceuticals, biochemicals, and biofuels. Therefore, to improve the production of valuable metabolites, many investigations have been performed by focusing on CRISPR-Cas- based metabolic engineering of this host. In the current review, the biology underlying CRISPR-Cas9 system was briefly explained and then the applications of CRISPR-Cas9/CRISPRi tools were considered for cell factory construction in E. coli.

中文翻译：

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用于大肠杆菌细胞工厂构建的 CRISPR–Cas9/CRISPRi 工具

创新的基于 CRISPR-Cas 的基因组编辑技术提供了一些功能和优势，例如高效率和特异性以及易于处理。CRISPR-Cas9系统的基因工程和基因调控两个方面都可以有利地应用于微生物细胞工厂的建设。作为使用最广泛的细胞工厂之一，大肠杆菌已被设计用于生产各种高附加值的化合物，如药物、生物化学品和生物燃料。因此，为了提高有价值的代谢物的产生，已经通过关注该宿主的基于 CRISPR-Cas 的代谢工程进行了许多研究。在目前的审查中，