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Ki-67 "hot spot" digital analysis is useful in the distinction of hepatic adenomas and well-differentiated hepatocellular carcinomas.
Virchows Archiv ( IF 3.4 ) Pub Date : 2020-06-25 , DOI: 10.1007/s00428-020-02868-8
Andrea Jones 1 , Trynda N Kroneman 2 , Anthony J Blahnik 2 , Rondell P Graham 3 , Taofic Mounajjed 3 , Michael S Torbenson 3 , Roger K Moreira 3
Affiliation  

This study aims to investigate the utility of digital protocols for Ki-67 immunohistochemistry quantitative analysis (“hot spot” method) in the setting of well-differentiated hepatocellular neoplasms. Resection cases of typical hepatic adenomas (HAs) (n = 40), atypical HAs (n = 9), and well-differentiated hepatocellular carcinomas (WD HCCs) (n = 56) were selected. HAs were further classified by immunohistochemistry using antibodies against liver fatty acid binding protein, glutamine synthetase, B-catenin, hepatic serum amyloid A, and C-reactive protein. Ki-67 proliferative index by immunohistochemistry was evaluated in all cases by digital analysis using a modified neuroendocrine tumor “hot spot” protocol. The proliferative rate of HAs (typical, median 1.2% (range 0–7.4%) and atypical, median 1.0% (range 0.3–3%)) was significantly lower than that of WD HCCs (median 4.5%, range 0–49.8%) (P < 0.0001). Only a few (7.5%) of the adenomas (all inflammatory/telangiectatic type) had proliferative rates higher than 4%, compared to most (51%) of HCCs. Ki-67 is a potentially useful adjunct marker in the evaluation of WD hepatocellular neoplasms, as “hot spot” proliferative rates are consistently very low in HAs but vary significantly in WD HCCs.



中文翻译:

Ki-67“热点”数字分析可用于区分肝腺瘤和高分化肝细胞癌。

这项研究的目的是研究数字化协议用于Ki-67免疫组织化学定量分析(“热点”方法)在高度分化的肝细胞肿瘤中的作用。典型肝腺瘤(n  = 40),非典型性 肝癌(n = 9)和高分化肝细胞癌(WD HCC)的切除病例(n = 56)。使用针对肝脏脂肪酸结合蛋白,谷氨酰胺合成酶,B-catenin,肝血清淀粉样蛋白A和C反应蛋白的抗体,通过免疫组织化学进一步对HA进行分类。使用改良的神经内分泌肿瘤“热点”方案,通过数字分析在所有情况下通过免疫组织化学评估Ki-67增殖指数。HAs的增殖率(典型值,中位数1.2%(范围0–7.4%)和非典型值,中位数1.0%(范围0.3–3%))显着低于WD HCCs(中位数4.5%,范围0–49.8%)。 )(P <0.0001)。与大多数(51%)肝癌相比,仅有少数(7.5%)腺瘤(所有炎症/毛细血管扩张型)的增生率高于4%。Ki-67是评估WD肝细胞肿瘤的潜在有用辅助标记物,因为HA的“热点”增殖率一直很低,而WD HCC则有很大差异。

更新日期:2020-06-25
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