This research screened for α-amylase inhibitory activity of twenties-five varieties Thai indigenous rice seeds. Based on specific inhibition, crude protein of var. Gai Ngaw (Gs. No. 13719) was selected for purification. The unbound proteins of the Q-Sepharose column named partially purified rice α-amylase inhibitor (RAI) revealed MW of approximately 14.4 kDa. The RAI was stable at pH 4 to 7 and heat stable up to 80 °C. The RAI had IC₅₀ of 15.92 ± 1.08 µg/ml. The double reciprocal plot implied a mixed-type inhibitor. The Dixon and Cornish-Bowden plots were used to estimate Ki and αKi. This suggested Thai indigenous rice seeds could potentially be developed as a food supplement for blood sugar and weight controls.

中文翻译：

---

具有强效 α-淀粉酶抑制活性的糯米 (Oryza sativa L.) 蛋白质提取物。

本研究筛选了 25 个品种的泰国本土水稻种子的 α-淀粉酶抑制活性。基于特异性抑制，var的粗蛋白。选择 Gai Ngaw (Gs. No. 13719) 进行纯化。Q-Sepharose 柱的未结合蛋白命名为部分纯化的水稻 α-淀粉酶抑制剂 (RAI)，其分子量约为 14.4 kDa。RAI 在 pH 值 4 至 7 下稳定，热稳定性高达 80 °C。RAI 的 IC ₅₀为 15.92 ± 1.08 µg/ml。双倒数图暗示混合型抑制剂。Dixon 和 Cornish-Bowden 图用于估计K i 和 α K i。这表明泰国本土水稻种子有可能被开发为血糖和体重控制的食品补充剂。