The green alga Tetraspora sp. CU2551 was previously investigated and showed enhanced H₂ production under anaerobic (Ar purged) conditions by cells immobilized in a calcium alginate matrix (Maswanna et al., Biomass Bioenergy 111:88–95, 2018). Here, we report successful H₂ production in entrapped cells under aerobic conditions. The most favorable immobilization condition observed was 4% (w/v) final alginate concentration after gelation, 2.80- to 3.35-mm beads, and a biomass content of 0.125 mg DW mL⁻¹ alginate. H₂ production increased when the immobilized cells were incubated in S-deprived media which could be repeated up to six times when using refreshed media. After six cycles, the H₂ production reached 12.8 ± 0.9 mL H₂ 25 mL⁻¹ of medium, corresponding to a rate of 182 ± 20 nmol mg⁻¹ DW h⁻¹, which was significantly higher than previously observed for other microalgae. Thus, our results demonstrate a potential for photobiological H₂ production using immobilized Tetraspora sp. CU2551 cells, grown under ambient aerobic conditions.

绿藻Tetraspora sp。CU2551之前曾进行过研究，结果显示，固定在藻酸钙基质中的细胞在厌氧（Ar清除）条件下产生的H ₂产量增加（Maswanna等人，Biomass Bioenergy 111：88-95，2018）。在这里，我们报告了在有氧条件下在捕获的细胞中成功产生了H ₂。观察到的最有利的固定条件是凝胶化后最终藻酸盐浓度为4％（w / v），2.80-3.35 mm珠粒，生物质含量为0.125 mg DW mL ^-1藻酸盐。当将固定化细胞在S缺失的培养基中孵育时，H ₂产生增加，当使用刷新的培养基时，H _2的产量最多可重复六次。六个周期后，H ₂培养基的产量达到12.8±0.9 mL H ₂ 25 mL ^-1，对应的速率为182±20 nmol mg ^-1 DW h ^-1，这显着高于以前在其他微藻中观察到的速率。因此，我们的结果证明了使用固定化的Tetraspora sp生产光生物H ₂的潜力。CU2551细胞，在环境有氧条件下生长。