Strawberry (Fragaria × ananassa Duch.) is an important fruit in the food industry. Recently, several strawberry mutant genotypes with improved agronomic traits have been developed using gamma irradiation and somaclonal variation mutagenesis. This study was conducted to examine the genetic diversity and variation among 19 novel strawberry genotypes and 3 commercial strawberry cultivars using single nucleotide polymorphism (SNP) markers generated from genotyping-by-sequencing (GBS) analysis. In addition, the bio-functional compounds of the various strawberry genotypes were examined to determine the fruit’s potential for additional uses in the food industry. Using GBS sequencing data, a total of 506,916,648 clean data reads were generated, with 376,723,429 (74.3%) mapped reads. A set of 55,733 filtered SNPs was used to perform a phylogenetic analysis, which showed that there were clear differences among the strawberry genotypes based on their original cultivar, except for two mutant genotypes. The ellagic acid levels of 22 strawberry genotypes ranged from 1.71 to 12.10 mg/100 g with an average of 4.36 mg/100 g. Pelargonidin-3-glucoside was the major anthocyanin in 17 strawberry genotypes; however, cyanidin-3-glucoside was the major anthocyanin in two purple flower genotypes. Anthocyanin pigments were not detectable in the three white fruit genotypes. This study demonstrated that the SNPs and functional compound content are an efficient tool for mutant screening and for the selection of elite genotypes in strawberry breeding programs.

草莓（草莓 ×  ananassaDuch。）是食品工业中的重要水果。最近，利用γ射线辐照和体细胞克隆变异诱变技术开发了几种具有改良农艺性状的草莓突变体基因型。这项研究进行了遗传多样性和变异之间的19种草莓基因型和3个商业草莓品种使用单核苷酸多态性（SNP）标记，通过基因分型（GBS）分析产生的遗传多样性和变异。此外，还检查了各种草莓基因型的生物功能化合物，以确定该水果在食品工业中的潜在用途。使用GBS测序数据，总共生成506,916,648个干净数据读取，其中有376,723,429（74.3％）个映射读取。一组55733个已过滤的SNP用于进行系统发育分析，结果表明，除了两个突变体基因型，草莓基因型在其原始栽培种之间存在明显差异。22种草莓基因型的鞣花酸水平为1.71至12.10 mg / 100 g，平均为4.36 mg / 100 g。Pelargonidin-3-glucoside是17个草莓基因型中的主要花色苷。然而，花青素-3-葡萄糖苷是两种紫色花基因型中的主要花色苷。在三种白色水果基因型中均未检测到花青素色素。这项研究表明，SNP和功能性化合物的含量是用于草莓育种程序中的突变体筛选和精英基因型选择的有效工具。22种草莓基因型的鞣花酸水平为1.71至12.10 mg / 100 g，平均为4.36 mg / 100 g。Pelargonidin-3-glucoside是17个草莓基因型中的主要花色苷。然而，花青素-3-葡萄糖苷是两种紫色花基因型中的主要花色苷。在三种白色水果基因型中均未检测到花青素色素。这项研究表明，SNP和功能性化合物的含量是用于草莓育种程序中的突变体筛选和精英基因型选择的有效工具。22种草莓基因型的鞣花酸水平为1.71至12.10 mg / 100 g，平均为4.36 mg / 100 g。Pelargonidin-3-glucoside是17个草莓基因型中的主要花色苷。然而，花青素-3-葡萄糖苷是两种紫色花基因型中的主要花色苷。在三种白色水果基因型中均未检测到花青素色素。这项研究表明，SNP和功能性化合物的含量是用于草莓育种程序中的突变体筛选和精英基因型选择的有效工具。在三种白色水果基因型中均未检测到花青素色素。这项研究表明，SNP和功能性化合物的含量是用于草莓育种程序中的突变体筛选和精英基因型选择的有效工具。在三种白色水果基因型中均未检测到花青素色素。这项研究表明，SNP和功能性化合物的含量是用于草莓育种程序中的突变体筛选和精英基因型选择的有效工具。