The native cartilage extracellular matrix (ECM) is enriched in sulfated glycosaminoglycans with important roles in the signaling and phenotype of resident chondrocytes. Recapitulating the key ECM components within engineered tissues through biomimicking strategies has potential to improve the regenerative capacity of encapsulated cells and lead to better clinical outcome. Here, we developed a double-modified, biomimetic and tissue adhesive hydrogel for cartilage engineering. We demonstrated sequential modification of alginate with first sulfate moieties to mimic the high glycosaminoglycan content of native cartilage and then tyramine moieties to allow in situ enzymatic crosslinking with tyrosinase under physiological conditions. Tyrosinase-crosslinked alginate sulfate tyramine (ASTA) hydrogels showed strong adhesion to native cartilage tissue with higher bond strength compared to alginate tyramine (AlgTA). Both ASTA and AlgTA hydrogels supported the viability of encapsula...

中文翻译：

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酪氨酸酶交联、组织粘合剂和仿生海藻酸盐硫酸盐水凝胶用于软骨修复。

天然软骨细胞外基质 (ECM) 富含硫酸化糖胺聚糖，在常驻软骨细胞的信号传导和表型中具有重要作用。通过仿生策略重述工程组织内的关键 ECM 成分有可能提高封装细胞的再生能力并导致更好的临床结果。在这里，我们开发了一种用于软骨工程的双重修饰、仿生和组织粘附水凝胶。我们展示了先用硫酸盐部分对海藻酸盐进行顺序修饰，以模拟天然软骨的高糖胺聚糖含量，然后用酪胺部分，在生理条件下与酪氨酸酶进行原位酶交联。酪氨酸酶交联的海藻酸盐硫酸酪胺 (ASTA) 水凝胶与海藻酸盐酪胺 (AlgTA) 相比，对天然软骨组织的粘附力更强，粘合强度更高。ASTA 和 AlgTA 水凝胶都支持封装的可行性...