In this Letter, we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics are implemented by the introduction of a multiactuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom-made multiphoton excitation microscope.

中文翻译：

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用于基于自适应透镜的商用激光扫描荧光显微镜的即插即用自适应光学器件。

在这封信中，我们提出了在现有的任何激光扫描荧光显微镜中简单实现自适应光学的解决方案。通过在显微镜主体和物镜之间引入多驱动器自适应透镜来实现自适应光学器件。通过优化显微镜软件在屏幕上显示的图像质量，可以使用无传感器方法进行校正。我们介绍了在商业线性激发共聚焦显微镜和定制的多光子激发显微镜上获得的结果。