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Nonenzyme Cascaded Amplification Biosensor Based on Effective Aggregation Luminescence Caused by Disintegration of Silver Nanoparticles.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-06-24 , DOI: 10.1021/acssensors.9b02355 Weipan Peng 1 , Yi Qin 1 , Wenna Li 1 , Minghui Chen 1 , Dianming Zhou 2 , Hengxuan Li 1 , Jingyu Cui 1 , Jin Chang 1 , Sheng Xie 3 , Xiaoqun Gong 1 , Benzhong Tang 4, 5
ACS Sensors ( IF 8.2 ) Pub Date : 2020-06-24 , DOI: 10.1021/acssensors.9b02355 Weipan Peng 1 , Yi Qin 1 , Wenna Li 1 , Minghui Chen 1 , Dianming Zhou 2 , Hengxuan Li 1 , Jingyu Cui 1 , Jin Chang 1 , Sheng Xie 3 , Xiaoqun Gong 1 , Benzhong Tang 4, 5
Affiliation
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Sensitive and portable quantification of biomarkers has particular significance in the monitoring and treatment of clinical diseases. Conventional immunoassays were accustomed to introducing or incorporating enzymes for signal amplification, which commonly suffered from poor stability and inferior tolerance. Herein, we constructed a novel nonenzyme amplification methodology based on fluorogenic Ag+-tetrazolate aggregation coupled with silver corrosion sensitization for biomarker determination. A significant cascade enhancement strategy was achieved by the valid aggregation luminescence caused by the potent disintegration of silver nanoparticles. Furthermore, efficient magnetic separation was also combined and performed for the rapidity and simplicity of operation. As the target, the detection limit of prostate-specific antigen was 15.66 pg/mL in our designed biosensor. Besides, a good linear relationship was obtained. The designed biosensor demonstrated good specificity and was successfully applied to clinical serum sample detection.
中文翻译：
基于银纳米颗粒崩解引起的有效聚集发光的非酶级联扩增生物传感器。
生物标志物的灵敏和便携式定量在临床疾病的监测和治疗中具有特别重要的意义。常规的免疫测定习惯于引入或掺入用于信号放大的酶，这些酶通常具有较差的稳定性和较差的耐受性。在这里，我们构建了一种基于荧光Ag +的新型非酶扩增方法-四唑酸盐聚集与银腐蚀敏化结合用于生物标志物的测定。有效的聚集发光是由银纳米颗粒强力崩解引起的，从而实现了重要的级联增强策略。此外，还结合并执行了有效的磁分离，以实现操作的快速性和简便性。作为目标，在我们设计的生物传感器中，前列腺特异性抗原的检出限为15.66 pg / mL。此外，获得了良好的线性关系。设计的生物传感器表现出良好的特异性，并成功应用于临床血清样品检测。
更新日期：2020-07-24
中文翻译：
基于银纳米颗粒崩解引起的有效聚集发光的非酶级联扩增生物传感器。
生物标志物的灵敏和便携式定量在临床疾病的监测和治疗中具有特别重要的意义。常规的免疫测定习惯于引入或掺入用于信号放大的酶，这些酶通常具有较差的稳定性和较差的耐受性。在这里，我们构建了一种基于荧光Ag +的新型非酶扩增方法-四唑酸盐聚集与银腐蚀敏化结合用于生物标志物的测定。有效的聚集发光是由银纳米颗粒强力崩解引起的，从而实现了重要的级联增强策略。此外，还结合并执行了有效的磁分离，以实现操作的快速性和简便性。作为目标，在我们设计的生物传感器中，前列腺特异性抗原的检出限为15.66 pg / mL。此外，获得了良好的线性关系。设计的生物传感器表现出良好的特异性，并成功应用于临床血清样品检测。
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