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Text-Displaying Semiquantitative Competitive Lateral Flow Immunoassay Relying on Inkjet-Printed Patterns.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-06-23 , DOI: 10.1021/acssensors.0c00637
Kazushi Misawa 1 , Tomohiro Yamamoto 2 , Yuki Hiruta 1 , Hiroki Yamazaki 2 , Daniel Citterio 1
Affiliation  

This work describes a colorimetric signaling approach for competitive lateral flow immunoassays (LFIAs) enabling sensitive and semiquantitative direct visual result readout in the form of “text”, demonstrated on the example of 8-hydroxy-2′-deoxyguanosine (8-OHdG) detection. The distinctive feature of the developed text-displaying LFIA (TD-LFIA) is the test zone system consisting of a combination of two types of inkjet-deposited capture molecules referred to as “mask antigen” and “text antibody”, allowing for sensitive turn-on signaling as opposed to the inverse response of conventional competitive LFIAs. The user operation is limited to sample application, followed by direct reading of assay results written in text after approximately 10 min. TD-LFIAs enabled the visual detection of 8-OHdG at concentrations down to 3 ng/mL, which is a 2–3 orders of magnitude lower visual detection limit than that achieved with the corresponding conventional design and is comparable to the existing LFIAs relying on external signal readout equipment. Highly reproducible observer-independent assay performance was confirmed, and the result interpretation is not influenced by sample color and readout timing. Making use of customizable threshold settings for text appearance, a device for semiquantitative assays was developed and successfully applied to the detection of 8-OHdG at four concentration levels (trace, low, medium, and high) in 54 human urine samples within the clinically relevant concentration range. The sensitive and intuitive signaling method of the developed system offers great potential for an alternative competitive LFIA platform suitable for real-world point-of-care testing applications.

中文翻译:

依靠喷墨打印图案的文本显示半定量竞争性横向流免疫分析。

这项工作描述了一种用于竞争性侧向流免疫测定(LFIA)的比色信号方法,能够以“文本”形式进行灵敏和半定量的直接视觉结果读出,在8-hydroxy-2'-deoxyguanosine(8-OHdG)检测示例中得到了证明。研发的文本显示LFIA(TD-LFIA)的显着特征是测试区系统,该系统由两种类型的喷墨沉积捕获分子(称为“面膜抗原”和“文本抗体”)的组合组成,可实现灵敏的转向-on信令,而不是传统竞争性LFIA的逆响应。用户操作仅限于样品应用,然后大约10分钟后直接读取以文本形式写入的测定结果。TD-LFIAs可以视觉检测低至3 ng / mL的8-OHdG,与相应的常规设计相比,其视觉检测极限低2-3个数量级,并且与依靠外部信号读取设备的现有LFIA相当。证实了高度可重复的独立于观察者的测定性能,并且结果的解释不受样品颜色和读出时间的影响。利用可定制的文本显示阈值设置,开发了一种用于半定量分析的设备,该设备已成功应用于临床相关范围内的54个人类尿液样品中四种浓度水平(痕量,低,中和高)的8-OHdG检测浓度范围。
更新日期:2020-07-24
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