Abstract The purpose of this study was to examine the effect of adding an essential fatty acid mixture (EOM; Eucalyptus globulus Labill, thymus vulgaris, Cymbopogon nardus, and Syzygium aromaticum) to the drinking water in the heat stress broilers on the adipokine (Apelin, BDNF) response, the histopathologic changes in liver and intestines, and some biochemical parameters. This study lasted for a total of 42 days, including the physical exercise period (7 days) and the fattening period (35 days). A total of 400 Ross 308 male broilers (1-d-old) were randomly divided into 8 groups (50 animals per group), each of which was exposed to various conditions of temperature (C: 22 °C and SC: 36 °C) and treatment dose (C, 250, 500 and 750 mL/1000 L). Each group was divided into 5 subgroups, each comprising 10 animals. In the stress-free groups, whereas the Apelin level linearly decreased, the BDNF level linearly increased. In histopathology, the liver tissue was found to be normal in all groups whereas the duodenum villi length was found to increase in the group of 750 mL/1000 L. No statistically significant difference was found between the stressed groups and the non-stressed groups in terms of VLDL, Glucose, Total bilirubin, ALT, and TG (p > .05). While Apelin level increased in the stressed groups, the BDNF level increased in the group of 250 mL/1000 L. In the histopathological examination, a small amount of coagulation necrosis was detected in hepatocyte, a diffuse hydropic degeneration was observed in hepatocytes, and finally a dilatation and hyperaemia were seen in sinusoid in the groups of EOM-500 mL/1000 L and EOM-750 m/1000 L compared to the control group. Whereas there was no difference between the group of EOM-250 mL/1000 L and the control group in terms of duodenum villa length, there was a significant decrease in other groups (p < .00). In conclusion, this study showed that adding 250 mL/1000 L of EOM to the drinking water had a positive effect on the serum levels of Apelin and p-BDNF in the groups exposed to stress (p <.05). HIGHLIGHTS Heat stress results in serious annual economic losses in the poultry industry. Essential oil fatty mix acids have positive effects on poultry health. Apelin and BDNF may be a protective factor against deleterious consequences of chronic exposition to heat stress

中文翻译：

---

在热应激肉鸡的饮用水中添加必需脂肪酸混合物对脂肪因子（Apelin，BDNF）反应、肝脏和肠道组织病理学发现以及一些血液参数的影响

摘要 本研究的目的是检查在热应激肉鸡的饮用水中添加必需脂肪酸混合物（EOM；蓝桉、胸腺、香茅和香蒲）对脂肪因子（Apelin， BDNF) 反应、肝脏和肠道的组织病理学变化以及一些生化参数。本次研究共持续42天，包括体育锻炼期（7天）和育肥期（35天）。将400只Ross 308雄性肉鸡（1日龄）随机分为8组（每组50只），每组暴露于各种温度条件（C：22°C和SC：36°C） ) 和治疗剂量 (C、250、500 和 750 mL/1000 L)。每组分为5个亚组，每个亚组包括10只动物。在无压力组中，而 Apelin 水平线性下降，BDNF 水平线性增加。在组织病理学上，750 mL/1000 L 组发现所有组的肝组织均正常，而发现十二指肠绒毛长度增加。 应激组和非应激组之间无统计学差异。 VLDL、葡萄糖、总胆红素、ALT 和 TG（p > .05）。应激组Apelin水平升高，250 mL/1000 L组BDNF水平升高。 组织病理学检查，肝细胞有少量凝固性坏死，肝细胞出现弥漫性水肿变性，最终与对照组相比，EOM-500 mL/1000 L 和 EOM-750 m/1000 L 组的血窦可见扩张和充血。EOM-250 mL/1000 L 组与对照组在十二指肠别墅长度方面没有差异，而其他组则显着降低（p < .00）。总之，这项研究表明，在饮用水中添加 250 mL/1000 L 的 EOM 对暴露于压力的组的 Apelin 和 p-BDNF 的血清水平有积极影响（p <.05）。亮点 热应激导致家禽业每年遭受严重的经济损失。精油脂肪酸混合酸对家禽健康有积极影响。Apelin 和 BDNF 可能是防止长期暴露于热应激的有害后果的保护因素 该研究表明，在饮用水中添加 250 mL/1000 L 的 EOM 对暴露于压力的组的 Apelin 和 p-BDNF 的血清水平有积极影响（p <.05）。亮点 热应激导致家禽业每年遭受严重的经济损失。精油脂肪酸混合酸对家禽健康有积极影响。Apelin 和 BDNF 可能是防止长期暴露于热应激的有害后果的保护因素 该研究表明，在饮用水中添加 250 mL/1000 L 的 EOM 对暴露于压力的组的 Apelin 和 p-BDNF 的血清水平有积极影响（p <.05）。亮点 热应激导致家禽业每年遭受严重的经济损失。精油脂肪酸混合酸对家禽健康有积极影响。Apelin 和 BDNF 可能是防止长期暴露于热应激的有害后果的保护因素