ABSTRACT

Background

Rheumatoid arthritis (RA) is an autoinflammatory and self-perpetuating disease with both articular and extra-articular manifestations, such as cardiovascular complications, which are the leading cause of mortality and morbidity in RA patients. Impaired sugar and lipid metabolism are considered as the critical risk factors for cardiovascular disease (CVD). Regarding the regulatory function of Raptor in the immunometabolism, in this study, we evaluated the association between plasma sugar and lipid profiles with the gene expression of Raptor and the cytokine tumor necrosis factor-α (TNF-α), as an inflammatory mediator, in peripheral blood leukocyte of RA patients.

Material and methods

Thirty-five RA patients who received combinational disease modified anti-rheumatoid drugs (DMARD) regimen and thirty healthy subjects enrolled in this study. The gene expression of Raptor was assessed by the real-time PCR method, and the Plasma levels of glucose and lipids, as well as TNF-α, were obtained using Hitachi device and enzyme-linked immunosorbent assay (ELISA) technique, respectively.

Results

The gene expression of Raptor was reduced significantly in RA patients compared to the healthy subjects (p = .001). The plasma level of HDL was significantly higher in RA patients than the control group (p = .001), while the plasma level of LDL was reduced significantly in these patients (p = .001).

Conclusion

In our study, the reduced gene expression of Raptor may contribute to the impaired immunometabolism in RA patients, which is independent of plasma sugar and lipid profile.

中文翻译：

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类风湿性关节炎患者血浆糖和脂质谱与 mTOR (Raptor) 调节蛋白基因表达的关联

摘要

背景

类风湿关节炎 (RA) 是一种自身炎症和自我延续的疾病，具有关节和关节外表现，例如心血管并发症，这是 RA 患者死亡和发病的主要原因。糖和脂质代谢受损被认为是心血管疾病（CVD）的关键危险因素。关于 Raptor 在免疫代谢中的调节功能，在本研究中，我们评估了血浆糖和脂质谱与 Raptor 的基因表达和作为炎症介质的细胞因子肿瘤坏死因子-α (TNF-α) 之间的关联。 RA患者的外周血白细胞。

材料与方法

35 名接受联合疾病改良抗类风湿药物 (DMARD) 方案的 RA 患者和 30 名健康受试者参加了这项研究。采用实时荧光定量 PCR 方法检测 Raptor 基因表达，采用日立仪器和酶联免疫吸附试验 (ELISA) 技术分别获得血浆葡萄糖和脂质以及 TNF-α 水平。

结果

与健康受试者相比，RA 患者的 Raptor 基因表达显着降低 ( p = .001)。RA 患者的血浆 HDL 水平显着高于对照组 ( p = .001)，而这些患者的血浆 LDL 水平显着降低 ( p = .001)。

结论

在我们的研究中，Raptor 基因表达的降低可能导致 RA 患者的免疫代谢受损，这与血浆糖和脂质谱无关。