The adipose tissue-derived stromal vascular fraction (SVF) is a promising candidate for use in cell therapy and tissue engineering due to its regenerative and immunomodulatory properties. Some therapies are based on using the complete SVF product, whereas others depend on the expansion of adipose-derived stromal cells (ASCs) in culture. The latter application often involves a time delay between adipose tissue harvest and SVF isolation. This study investigated how storage time and temperature affected cell quality and composition. Aliquots of lipoaspirate were stored cold (4°C), at room temperature (18–20°C), or at 37°C. SVF was isolated on sequential time points over a period of 48 h, and the following were assessed: cell viability, vitality, composition, and the proliferative potential of the ASCs. When the lipoaspirate was stored cold, the viability of the SVF remained stable for up to 48 h; however, the vitality of the SVF decreased significantly after 24 h. When stored at higher temperatures (room temperature or 37°C), the vitality of the SVF decreased after 8 h. The ASC fraction in the SVF decreased rapidly after 8 h when stored at higher temperatures, whereas this change was delayed significantly when the lipoaspirate was stored cold. Tendencies towards increases in the lag phase, population doubling time (PDt), and time to reach confluency were observed when the lipoaspirate was stored at higher temperatures. The vitality of the SVF was correlated significantly with the time of the lag phase and the time required to reach confluence, whereas no correlation was observed with the PDt. Both prolonged storage time and increased temperature during lipoaspirate storage negatively affected the quality of the obtained SVF. Our results suggest that lipoaspirate should be stored for no longer than 24 h at 4°C to maintain the optimal quality for the isolation of SVF and the expansion of ASCs.

中文翻译：

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脂肪抽吸物储存时间和温度：对基质血管组分质量和细胞组成的影响

由于其再生和免疫调节特性，脂肪组织来源的基质血管部分 (SVF) 是用于细胞治疗和组织工程的有希望的候选者。一些疗法基于使用完整的 SVF 产品，而其他疗法则依赖于培养中脂肪衍生基质细胞 (ASC) 的扩增。后一种应用通常涉及脂肪组织收获和 SVF 分离之间的时间延迟。本研究调查了储存时间和温度如何影响细胞质量和组成。脂肪抽吸物的等分试样在低温 (4°C)、室温 (18–20°C) 或 37°C 下储存。在 48 小时内的连续时间点上分离 SVF，并评估以下内容：细胞活力、活力、组成和 ASC 的增殖潜力。当脂肪抽吸物冷藏时，SVF 的活力在长达 48 小时内保持稳定；然而，SVF的活力在24小时后显着下降。当储存在较高温度（室温或 37°C）时，SVF 的活力在 8 小时后下降。当储存在较高温度下 8 小时后，SVF 中的 ASC 分数迅速下降，而当脂肪抽吸物冷储存时，这种变化显着延迟。当脂肪抽吸物在较高温度下储存时，观察到滞后期、群体倍增时间 (PDt) 和达到汇合时间的增加趋势。SVF 的活力与滞后期的时间和达到汇合所需的时间显着相关，而与 PDt 没有相关性。在脂肪抽吸物储存期间延长的储存时间和升高的温度都会对获得的 SVF 的质量产生负面影响。我们的结果表明，脂肪抽吸物应在 4°C 下储存不超过 24 小时，以保持分离 SVF 和扩增 ASC 的最佳质量。