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The structural studies of a polysaccharide purified from Oat Lao‐Chao
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-06-23 , DOI: 10.1111/ijfs.14690 Rong A 1 , Meili Zhang 1 , Yu Lu 1 , Huijie Zhang 1 , Xue Bai 1
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2020-06-23 , DOI: 10.1111/ijfs.14690 Rong A 1 , Meili Zhang 1 , Yu Lu 1 , Huijie Zhang 1 , Xue Bai 1
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Oat Lao‐Chao contains bioactive components such as polysaccharides, of which immunomodulatory activity is recognised as the most important biological activity of polysaccharides. This paper studied optimisation of manufacturing Oat Lao‐Chao, extraction of polysaccharide and removal of its protein, separation and purification of polysaccharide. The results showed that a water‐soluble polysaccharide coded as LCPS‐2 was isolated from Oat Lao‐Chao. The polysaccharide was homogeneous and produced an asymmetrical peak obtained by high‐performance gel permeation chromatography. Monosaccharide analysis revealed that LCPS‐2 was containing arabinose, xylose, galactose, glucose and mannose in a ratio of 1.01: 0.72: 0.76: 0.28: 0.10. The GC‐MS results of the derived alditol acetates from the permethylated LCPS‐2 showed some separate peaks corresponding to (→6)‐ Galp,(→3,6)‐Galp, terminal Ara, (→5)‐Ara, (→1)‐linked Xyl, (→3)‐Xyl and (→6)‐Glc units. NMR analysis and acid hydrolysis experiments indicated that the core structure features include a chain consisting of (1,3,6)‐linked Galp, (1,4)‐linked Galp, (1,5)‐linked Ara residues.
中文翻译：
燕麦老巢提纯多糖的结构研究
燕麦老巢含有生物活性成分，例如多糖，其免疫调节活性被认为是多糖最重要的生物活性。本文研究了燕麦老巢的生产优化，多糖的提取和蛋白质的去除，多糖的分离与纯化。结果表明，从燕麦老巢分离出了编码为LCPS-2的水溶性多糖。多糖是均质的，并通过高效凝胶渗透色谱得到不对称峰。单糖分析显示LCPS-2含有阿拉伯糖，木糖，半乳糖，葡萄糖和甘露糖的比例为1.01：0.72：0.76：0.28：0.10。来自全甲基化LCPS-2的醛糖醇乙酸酯的GC-MS结果显示了一些单独的峰，对应于（→6）-Galp，（→3,6）-Galp，末端Ara，（→5）-Ara，（→ 1）-连接的Xyl，（→3）-Xyl和（→6）-Glc单元。NMR分析和酸水解实验表明，核心结构特征包括由（1,3,6）连接的Galp，（1,4）连接的Galp，（1,5）连接的Ara残基组成的链。
更新日期：2020-06-23
中文翻译：
燕麦老巢提纯多糖的结构研究
燕麦老巢含有生物活性成分，例如多糖，其免疫调节活性被认为是多糖最重要的生物活性。本文研究了燕麦老巢的生产优化，多糖的提取和蛋白质的去除，多糖的分离与纯化。结果表明，从燕麦老巢分离出了编码为LCPS-2的水溶性多糖。多糖是均质的，并通过高效凝胶渗透色谱得到不对称峰。单糖分析显示LCPS-2含有阿拉伯糖，木糖，半乳糖，葡萄糖和甘露糖的比例为1.01：0.72：0.76：0.28：0.10。来自全甲基化LCPS-2的醛糖醇乙酸酯的GC-MS结果显示了一些单独的峰，对应于（→6）-Galp，（→3,6）-Galp，末端Ara，（→5）-Ara，（→ 1）-连接的Xyl，（→3）-Xyl和（→6）-Glc单元。NMR分析和酸水解实验表明，核心结构特征包括由（1,3,6）连接的Galp，（1,4）连接的Galp，（1,5）连接的Ara残基组成的链。
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