Wound healing refers to a complex, dynamic process in the body, especially the skin, which influences the quality of life of millions of people each year. Electrospinning is an efficient and economic technique for the production of micro‐ to nano‐fibrillar constructs as drug delivery systems with biomedical applications. In this study, we designed an electrospun bilayer fibrillar scaffold immobilized with epidermal growth factor (EGF), using polycaprolactone (PCL) as the upper layer and chitosan (CS)/polyvinyl alcohol (PVA) as the lower layer of wound dressing. The scanning electron microscopy (SEM) was utilized to characterize the scaffolds. The water uptake, release potency, and toxicity of scaffolds were evaluated, and also then DAPI staining and MTT assay after seeding adipose tissue‐derived mesenchymal stem cells. Finally, the effects of the scaffolds on the wound healing process were investigated in a mouse model of full‐thickness skin injury. The results of SEM demonstrated continuous, smooth, bead‐free, and randomly aligned fibers. The average diameter of CS/PVA nanofibers was 238.36 ± 36.99, and the mean diameter of PCL nanofibers was 1271.79 ± 428.49 nm. The scaffolds were capable of cell seeding and supported their growth and proliferation. Furthermore, in the comparison between designed composite as dressing patch and control group (Vaseline), our composite demonstrated significant differences, so that EGF‐immobilized patch efficiently accelerated wound closure and improved histological healing in an in vivo full‐thickness wound healing mouse model. The present results suggest the potential of the designed electrospun bilayer fibrillar scaffold containing EGF for the treatment of full‐thickness wounds and skin regeneration.

中文翻译：

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含有表皮生长因子的双层纳米原纤维支架对全层伤口愈合的影响

伤口愈合是指身体（尤其是皮肤）中复杂而动态的过程，每年都会影响数百万人的生活质量。电纺丝是一种高效且经济的技术，可用于生产微纤维到纳米纤维的结构，并将其作为具有生物医学应用的药物输送系统。在这项研究中，我们设计了一种固定有表皮生长因子（EGF）的静电纺丝双层原纤维支架，使用聚己内酯（PCL）作为上层，壳聚糖（CS）/聚乙烯醇（PVA）作为伤口敷料的下层。利用扫描电子显微镜（SEM）表征支架。评估支架的吸水率，释放力和毒性，然后在播种脂肪组织来源的间充质干细胞后进行DAPI染色和MTT分析。最后，在全层皮肤损伤的小鼠模型中研究了支架对伤口愈合过程的影响。扫描电镜的结果表明，纤维连续，光滑，无珠子且排列随机。CS / PVA纳米纤维的平均直径为238.36±36.99，PCL纳米纤维的平均直径为1271.79±428.49 nm。支架能够播种细胞并支持其生长和增殖。此外，在设计为敷料贴的复合材料与对照组（凡士林）之间的比较中，我们的复合材料显示出显着差异，因此在体内全厚度伤口愈合小鼠模型中，固定有EGF的贴剂可有效加速伤口闭合并改善组织学愈合。