Structural elucidation of selenocysteine insertion machinery of microalgal selenoprotein T and its transcriptional analysis,Biotechnology and Applied Biochemistry

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Structural elucidation of selenocysteine insertion machinery of microalgal selenoprotein T and its transcriptional analysis
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-06-24 , DOI: 10.1002/bab.1974
Ragini Reshma _{1,

2} , Sunitha Kumari _{1,

2} , Muthu Arumugam _{1,

2}

Affiliation

Essential trace element selenium in association with selenoproteins, which is found in almost all organisms except higher plants and fungi, is involved in various biological functions. Advancement in the field of whole genome sequencing and data analyzing bioinformatic tools led to the accumulation of genome information of organisms. However, selenoproteins are unique and it needs specialized genomics tool for its identification as well as characterization. In this study, the presence of selenoprotein T (SelT) from Scenedesmus quadricauda was shown for the first time with experimental evidence and compared with SelT of marine microalgae Nannochloropsis oceanica. Along with SelT, all the associated machineries required to synthesize the selenoproteins were also identified. Also, the present study tried to explicate the evolutionary relatedness of SelT of these two organisms with other known bacteria and eukaryotes. Transcript level analysis in S. quadricauda under endoplasmic reticulum stress showed a 1.2 ± 0.28-fold increase in SelT expression. Thus, it provided the first experimental evidence on SelT expression from microalgae.

中文翻译：

微藻硒蛋白T硒代半胱氨酸插入机制的结构解析及其转录分析

必需微量元素硒与硒蛋白结合，几乎存在于除高等植物和真菌外的所有生物体中，参与各种生物学功能。全基因组测序和数据分析生物信息学工具领域的进步导致生物体基因组信息的积累。然而，硒蛋白是独一无二的，它需要专门的基因组学工具来进行识别和表征。在这项研究中，首次通过实验证据显示了来自四尾栅藻的硒蛋白 T (SelT) 的存在，并与海洋微藻Nannochloropsis Oceanica 的 SelT 进行了比较。. 与 SelT 一起，还鉴定了合成硒蛋白所需的所有相关机器。此外，本研究试图解释这两种生物的 SelT 与其他已知细菌和真核生物的进化相关性。在转录水平分析S. quadricauda内质网应激下显示出SELT表达1.2±0.28倍。因此，它提供了关于来自微藻的 SelT 表达的第一个实验证据。

更新日期：2020-06-24

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