Measuring the growth rate of non‐model anaerobic microbes typically requires the use of time‐consuming and often destructive manual measurements. Here, an Arduino based automatic pressure evaluation system (A‐APES) was developed to automatically measure the rate of fermentation gas production as a proxy for microbial growth in anaerobic systems. The A‐APES system measures accumulated gas pressure in sealed cultures accurately at high‐resolution, while venting the system at programmed intervals to prevent over pressurization. The utility of A‐APES is demonstrated in this study by quantifying the growth rate and phases of a biomass‐degrading anaerobic gut fungus, which cannot be otherwise measured via conventional techniques due to its association with particulate substrates. Given the utility of the A‐APES approach, we provide a complete construction guide to fabricate the device, which is three times less expensive compared to existing commercial alternatives.

中文翻译：

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基于Arduino的自动压力评估系统，可量化文化中非模型厌氧菌的生长

测量非模型厌氧微生物的生长速度通常需要使用耗时且通常具有破坏性的手动测量。在这里，开发了基于Arduino的自动压力评估系统（A‐APES），以自动测量发酵气体的产生速率，作为厌氧系统中微生物生长的代理。A‐APES系统可在高分辨率下准确测量密封培养物中的累积气压，同时以编程的时间间隔对系统进行排气，以防止压力过大。通过量化生物量降解厌氧菌真菌的生长速率和阶段来证明A‐APES的效用，由于与颗粒基质的结合，传统方法无法通过其他方法对其进行测量。鉴于A‐APES方法的实用性，