Reverse genetics technology allows one to engineer replication-competent viruses from cloned cDNAs at will. Since the establishment of the initial reverse genetics system for species A rotaviruses (RVAs) requiring a helper virus in 2006, attempts have been successfully made to improve this technology. Efficient generation of replication-competent RVAs is now possible from just 11 T7-driven plasmids encoding an RVA genome when the quantity ratio of the two rescue T7-driven plasmids for the NSP2 and NSP5 segments is increased by 3-fold in relation to that of the other nine plasmids (11 plasmid-only system). Further, it is now possible to generate recombinant RVAs even with severely less efficient infectivity by using the 11 plasmid-only system, which has not been possible with the existing approaches. More importantly, the 11 plasmid-only system does not need any helper expression plasmid, and thus this simplest and robust system has a clear advantage over the existing systems in terms of safety. This 11 plasmid-only system should contribute to the development of safe next-generation vaccines and vaccine vectors.

逆向遗传学技术使人们可以从克隆的cDNA中随意工程改造具有复制能力的病毒。自2006年建立了需要辅助病毒的A类轮状病毒（RVA）的初始反向遗传学系统以来，已成功进行了改进该技术的尝试。现在，当NSP2和NSP5区段的两个抢救性T7驱动质粒的数量比相对于NSP2的3倍增加时，仅由11个编码RVA基因组的T7驱动质粒即可有效生成具有复制能力的RVA。其他9个质粒（仅11个质粒系统）。此外，现在可以通过使用仅11个质粒的系统来产生重组RVA，即使其感染效率大大降低，这是现有方法无法实现的。更重要的是，仅11个质粒的系统不需要任何辅助表达质粒，因此，在安全性方面，这种最简单，最可靠的系统明显优于现有系统。仅11个质粒的系统应有助于开发安全的下一代疫苗和疫苗载体。