Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.

人类 RNF2 (RING1B) 基因是胚胎干细胞 (ESC) 胚胎发育、多能性和分化的关键表观遗传修饰因子。为了进一步深入了解RNF2在人类ESC细胞命运决定中的作用，我们通过CRISPR/Cas9基因组编辑技术生成了两个RNF2纯合敲除人类ESC系。这些细胞系在形态、多能基因表达等方面均保持了正常的核型和典型的未分化状态，具有体内分化潜力。这些细胞系为探索RNF2基因在体外胚胎发育和谱系分化中的作用提供了良好的细胞资源。