Abstract Phenolic compounds are active compounds in Dendrobium formosum Roxb. ex Lindl. that have antidiabetic and anti-obesity activities. To obtain a high extraction yield of phenolic compounds from D. formosum, optimization of microwave-assisted extraction (MAE) was developed in this study using response surface methodology. The optimal conditions for phenolic compound yield were using absolute ethanol as the solvent, 540 W of microwave power, 49.37 s of extraction time, a 22.67 mL/g solvent-to-feed ratio, and three extraction cycles. The yields of coelonin, erianthridin, moscatilin, lusianthridin, gigantol and batatasin III when extracted under optimal conditions were 0.38 ± 0.04, 0.40 ± 0.05, 1.45 ± 0.18, 0.14 ± 0.01, 0.47 ± 0.05 and 0.58 ± 0.06 mg/g dry weight, respectively, with errors of less than 4% deviation from the predicted values. Our results indicate that MAE can obtain a higher yield of phenolic compounds and have a greater effect on glucose uptake than those of other extraction methods. Therefore, MAE has high efficiency for extracting phenolic compounds from D. Fomosum, yielding extracts with a high amount of compounds that exhibit antidiabetic activity.

中文翻译：

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石斛中酚类化合物的微波辅助提取工艺优化[J]. 前林德尔。和葡萄糖摄取活性

摘要 酚类化合物是石斛中的活性化合物。前林德尔。具有抗糖尿病和抗肥胖活性。为了从 D. formosum 中获得较高的酚类化合物提取率，本研究中使用响应面方法优化了微波辅助提取 (MAE)。酚类化合物收率的最佳条件是使用无水乙醇作为溶剂、540 W 微波功率、49.37 s 提取时间、22.67 mL/g 溶剂进料比和三个提取循环。在最佳条件下提取的 coelonin、erianthridin、moscatilin、lusianthridin、gigantol 和 batatasin III 的产量为 0.38 ± 0.04、0.40 ± 0.05、1.45 ± 0.18、0.14 ± 0.01、0.5 mg ±0.5 和 0.5 mg ±0.5分别与预测值的偏差小于 4%。我们的结果表明，与其他提取方法相比，MAE 可以获得更高的酚类化合物收率，并且对葡萄糖摄取的影响更大。因此，MAE 可以高效地从 D. Fomosum 中提取酚类化合物，产生具有大量具有抗糖尿病活性的化合物的提取物。