Food poisoning caused by Staphylococcus aureus (S. aureus) toxins is considered one of the foremost public health threat that usually occurs through the ingestion of raw milk contaminated with staphylococcal enterotoxins. The current study spotlights on the prevalence, antibiogram and genetic diversity of S. aureus enterotoxin genes. One hundred and fifty of raw milk (90) and ice cream (60) samples were randomly collected from local markets from Sadat city, Egypt. S. aureus was recovered from 44% of raw milk and 20% of ice cream samples. The identification for the obtained S. aureus isolates was confirmed through targeting the nuc gene. Antibiogram pattern of 32 S. aureus isolates showed high resistance to Cefoxitin, Sulpha/Trimethoprim, Tetracycline, Norfloxacin, Penicillin and Cephradine. However, high susceptibility to Gentamycin and Vancomycin were observed. Multiplex PCR was a competent practise for the recognition of Staphylococcus enterotoxin (SE) genes (SEA, SEB and SED). The phylogenetic analysis of the SED gene of enterotoxigenic S. aureus strains showed identical similarity with 100% to each other and high similarity with other international isolates in GenBank from different localities and sources. The frequency of enterotoxigenic S. aureus strains in milk products could have serious hazardous effects on humans. These results suggested possible strains transmission between different geographical areas through the food and milk product trades.

由金黄色葡萄球菌（金黄色葡萄球菌）毒素引起的食物中毒被认为是最重要的公共健康威胁之一，通常是由于摄入被葡萄球菌肠毒素污染的原奶而发生的。当前的研究聚焦于金黄色葡萄球菌肠毒素基因的流行，抗菌谱和遗传多样性。从埃及萨达特市的当地市场中随机收集了150份原奶（90）和冰淇淋（60）样品。从44％的原奶和20％的冰淇淋样品中回收金黄色葡萄球菌。通过靶向nuc基因，证实了对获得的金黄色葡萄球菌分离株的鉴定。抗菌图案32金黄色葡萄球菌分离株对头孢西丁，Sulpha /甲氧苄啶，四环素，诺氟沙星，青霉素和头孢拉定具有高抗性。然而，观察到对庆大霉素和万古霉素的高度敏感性。多重PCR是识别葡萄球菌肠毒素（SE）基因（SEA，SEB和SED）的有效方法。对产肠毒素的金黄色葡萄球菌菌株的SED基因进行系统发育分析，显示彼此之间100％相同，并且与GenBank中来自其他地区和来源的其他国际分离株高度相似。产肠毒素金黄色葡萄球菌的频率乳制品中的菌株可能对人类造成严重的危害。这些结果表明，可能通过食品和奶制品贸易在不同地理区域之间传播毒株。