Research question

Is GSTM1 and GSTT1 deletion associated with the development of polycystic ovary syndrome (PCOS)?

Design

A case-control study was designed to investigate the association between GSTM1 and GSTT1 gene polymorphisms with PCOS. Blood samples from 201 women diagnosed with infertility were taken, of which 69 women were diagnosed with PCOS. Genomic DNA was extracted, and genotyping analyses were conducted by polymerase chain reaction-based methods. Odds ratios and 95% confidence intervals were calculated by unconditional logistic regression.

Results

An increased risk of PCOS was found to be associated with GSTT1 null genotype (OR 4.890, 95% CI 2.261 to 9.122; P < 0.001). A strong association between GSTT1 null genotype was found with female infertility, regardless of the associated cause (OR 5.300, 95% CI 3.238 to 8.675; P < 0.001) as well as with the GSTM1 null genotype (OR 1.620, 95% CI 1.067 to 2.459; P = 0.026). A statistically significant association with the development of infertility was also found when carriers of the combined genotype GSTT1+/GSTM1+ was compared with carriers of the combined genotype GSTT1–/ GSTM1+ (OR 3.600 95% CI 1.864 to 6.956; P < 0.001). The two-way combination of GSTT1 and GSTM1 null genotypes resulted in an increased susceptibility to infertility development (OR 11.136; 95% CI 5.035 to 24.629; P < 0.001).

Conclusions

Carriers of GSTT1 null genotype seem to have higher susceptibility to developing PCOS and infertility from other causes. Also, GSTT1 null genotype, alone or in association, are related with increased susceptibility to infertility development, independently of its cause. GSTM1 null genotype is only associated with all cause of infertility when the GSTT1 is null.

中文翻译：

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患有多囊卵巢综合征和其他不孕症的女性具有更高的 GSTT1 缺失患病率。

研究问题

被GSTM1和GSTT1多囊卵巢综合征（PCOS）的发展相关联的缺失？

设计

一项病例对照研究旨在调查GSTM1和GSTT1基因多态性与 PCOS之间的关联。采集了 201 名被诊断为不孕症的女性的血液样本，其中 69 名女性被诊断出患有 PCOS。提取基因组 DNA，并通过基于聚合酶链反应的方法进行基因分型分析。通过无条件逻辑回归计算优势比和 95% 置信区间。

结果

发现 PCOS 风险增加与GSTT1无效基因型相关（OR 4.890，95% CI 2.261 至 9.122；P < 0.001）。无论相关原因如何（OR 5.300，95% CI 3.238 至 8.675；P < 0.001）以及GSTM1无效基因型（OR 1.620，95% CI 1.067 ），都发现GSTT1无效基因型与女性不育之间存在很强的关联2.459；P  = 0.026)。当组合基因型GSTT1 +/ GSTM1 + 的携带者与组合基因型GSTT1 –/ GSTM1 的携带者进行比较时，还发现了与不孕症的发展具有统计学意义的相关性+（OR 3.600 95% CI 1.864 至 6.956；P < 0.001）。GSTT1和GSTM1无效基因型的双向组合导致对不孕症的易感性增加（OR 11.136；95% CI 5.035 至 24.629；P < 0.001）。

结论

GSTT1无效基因型的携带者似乎对因其他原因发展为多囊卵巢综合征和不孕症的易感性更高。此外，GSTT1无效基因型，单独或联合，与增加的不孕症易感性相关，与其原因无关。当GSTT1为空时，GSTM1空基因型仅与不育的所有原因相关。