Abstract Synthesis of gold nanoparticles was carried out using Pongammia pinnata (pongam) leaf extract and their anticancer and antimycobacterial activities were studied. Gold nanoparticle formation was confirmed by UV–vis, XRD and HR-TEM. The anticancer efficacies of the biogenic gold nanoparticles were analyzed using cytotoxicity, cell morphology analysis, oxidative DNA damage, apoptosis detection and toxicity studies. Biogenic gold nanoparticles inhibited breast cancer cell line (MCF-7) proliferation with an efficacy of IC50 of 1.85 μg/mL. The antimycobacterial potential of the biogenic gold nanoparticles was screened against M. tuberculosis by Luciferase Reporter Phage (LRP) assay. The gold nanoparticles showed inhibition against sensitive M. tuberculosis with the minimum inhibitory concentration (MIC) of 10 μg/mL whereas no inhibition was found against the rifampicin resistant M. tuberculosis.

中文翻译：

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揭示生物工程金纳米粒子的抗癌和抗分枝杆菌潜力

摘要 以水杨（Pongamia pinnata）叶提取物为原料合成了金纳米粒子，并研究了其抗癌和抗分枝杆菌活性。UV-vis、XRD 和 HR-TEM 证实了金纳米颗粒的形成。使用细胞毒性、细胞形态分析、氧化DNA损伤、细胞凋亡检测和毒性研究来分析生物金纳米粒子的抗癌功效。生物金纳米颗粒抑制乳腺癌细胞系 (MCF-7) 增殖，IC50 为 1.85 μg/mL。通过荧光素酶报告基因噬菌体 (LRP) 测定法筛选了生物金纳米粒子的抗分枝杆菌潜力，以对抗结核分枝杆菌。金纳米颗粒显示出对敏感 M 的抑制作用。