Abstract In the last decades, the understanding of the complex biological mechanisms that regulate the life and growth of single cells and their reciprocal interaction has steadily increased. In this process, Quantitative Phase Imaging (QPI) has emerged as an invaluable tool, thus providing a new way of investigating live cell behaviour in a dynamic, label-free and non-invasive modality. It has proved especially relevant to biomedical and diagnostic applications, as the information-rich quantitative phase data opens new channels to investigate pathophysiology. In this review, we expose the recent developments in the extraction of unique biological information, and we provide a schematic overview of the currently developed techniques. Specifically, we identify four different trends of relevance in the context of biomedical applications, namely QPI-cytometry, development of point-of-care devices, tomographic phase reconstruction techniques and learning-based approaches, providing a current insight in the way they are shifting our approach to the acquisition and treatment of QPI data.

中文翻译：

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生物医学应用中的定量相位成像趋势

摘要 在过去的几十年里，对调节单细胞生命和生长及其相互作用的复杂生物学机制的理解稳步增加。在此过程中，定量相位成像 (QPI) 已成为一种宝贵的工具，从而提供了一种以动态、无标记和非侵入性方式研究活细胞行为的新方法。事实证明，它与生物医学和诊断应用特别相关，因为信息丰富的定量相位数据开辟了研究病理生理学的新渠道。在这篇评论中，我们揭示了提取独特生物信息的最新进展，并提供了当前开发技术的示意图概述。具体来说，我们确定了生物医学应用背景下的四种不同的相关趋势，