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Serotonin Expression in the Song Circuitry of Adult Male Zebra Finches.
Neuroscience ( IF 2.9 ) Pub Date : 2020-06-24 , DOI: 10.1016/j.neuroscience.2020.06.018
Ping K Yip 1 , Magdalena Schmitzberger 1 , Mohammed Al-Hasan 1 , Julia George 2 , Elina Tripoliti 3 , Adina T Michael-Titus 1 , David Clayton 2 , John V Priestley 1
Affiliation  

Serotonin is an important neurotransmitter of the brain, but its role in song control remains to be fully demonstrated. Using male zebra finches (Taeniopygia guttata) that have song learning and production capabilities, we analysed the serotonin expression levels in the song nuclei and adjacent areas (peri-song nuclei) using immunohistochemistry. Key song nuclei were identified using combinations of Hoechst, choline acetyltransferase, and a neurofilament (NN18) marker in reference to the ZEBrA atlas. Mean serotonin expression was highest in interfacial nucleus (Nif) and lower in the other song nuclei in the following order (in order of highest first): interfacial nucleus (Nif) > Area X > dorsomedial part of the intercollicular nucelus (DM) > robust nucleus of the archistriatum (RA) > lateral magnocellular nucleus of the anterior neostriatum (LMAN) > ventral respiratory group (VRG) > dorsolateral nucleus of the medial thalamus (DLM) > the nucleus HVC (proper name) > tracheosyringeal motor nucleus (nXIIts). However, the mean serotonin expression (in order of highest first) in the peri-song nuclei regions was: peri-DM > peri-nXIIts > supra-peri-HVC > peri-RA > peri-DLM > peri-Area X > infra-peri-HVC > peri-VRG > peri-LMAN > peri-Nif. Interestingly, serotoninergic fibers immunostained for serotonin or the serotonin transporter can be found as a basket-like peri-neuronal structure surrounding cholinergic cell bodies, and appear to form contacts onto dopaminergic neurones. In summary, serotonin fibers are present at discrete song nuclei, and peri-song nuclei regions, which suggest serotonin may have a direct and/or modulatory role in song control.



中文翻译:

成年男性斑马雀鸣曲中血清素的表达。

5-羟色胺是大脑的重要神经递质,但其在歌曲控制中的作用尚待充分证明。使用雄性斑马雀(Taeniopygia guttata)具有歌曲学习和制作功能,我们使用免疫组织化学分析了歌曲核及邻近区域(peri-song核)中5-羟色胺的表达水平。参考ZEBrA图谱,使用Hoechst,胆碱乙酰基转移酶和神经丝(NN18)标记的组合鉴定了关键的歌曲核。血清素的平均表达在界面核(Nif)中最高,而在其他歌曲核中则依次降低(按最高顺序排列):界面核(Nif)> X区域>交界面核的背侧部分(DM)>健壮足弓核(RA)>纹状体前外侧核细胞核(LMAN)>腹侧呼吸组(VRG)>内侧丘脑背外侧核(DLM)> HVC核(专有名称)> 气管注射器运动核(nXIIts)。然而,在周围歌曲的核区域中,血清素的平均表达量(从高到低的顺序为)为:peri-DM>周-nXIIts>周上-HVC>周-RA>周-DLM>周-区域X>下-peri-HVC> peri-VRG> peri-LMAN> peri-Nif 有趣的是,针对5-羟色胺或5-羟色胺转运蛋白免疫染色的5-羟色胺能纤维可以发现为胆碱能细胞体周围的篮状神经元周围结构,并似乎与多巴胺能神经元形成接触。总而言之,5-羟色胺纤维存在于离散的歌曲核和歌周核区域,这表明5-羟色胺可能在歌曲控制中具有直接和/或调节作用。上皮>上皮HVC>皮下RA>皮下DLM>皮下X>皮下HVC>上皮VRG>上皮LMAN>皮下Nif。有趣的是,针对5-羟色胺或5-羟色胺转运蛋白免疫染色的5-羟色胺能纤维可以发现为胆碱能细胞体周围的篮状神经元周围结构,并似乎与多巴胺能神经元形成接触。总而言之,5-羟色胺纤维存在于离散的歌曲核和歌周核区域,这表明5-羟色胺可能在歌曲控制中具有直接和/或调节作用。上皮>上皮HVC>皮下RA>皮下DLM>皮下X>皮下HVC>上皮VRG>上皮LMAN>皮下Nif。有趣的是,针对5-羟色胺或5-羟色胺转运蛋白免疫染色的5-羟色胺能纤维可以发现为胆碱能细胞体周围的篮状神经元周围结构,并似乎与多巴胺能神经元形成接触。总而言之,5-羟色胺纤维存在于离散的歌曲核和歌周核区域,这表明5-羟色胺可能在歌曲控制中具有直接和/或调节作用。对5-羟色胺或5-羟色胺转运蛋白免疫染色的5-羟色胺能纤维可发现为胆碱能细胞体周围的篮状神经元周围结构,似乎与多巴胺能神经元形成接触。总而言之,5-羟色胺纤维存在于离散的歌曲核和歌周核区域,这表明5-羟色胺可能在歌曲控制中具有直接和/或调节作用。对5-羟色胺或5-羟色胺转运蛋白免疫染色的5-羟色胺能纤维可以发现为胆碱能细胞体周围的篮状神经元周围结构,似乎与多巴胺能神经元形成接触。总而言之,5-羟色胺纤维存在于离散的歌曲核和歌周核区域,这表明5-羟色胺可能在歌曲控制中具有直接和/或调节作用。

更新日期:2020-08-30
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