Phytotoxins are widely found in plant pathogens. In recent years, many diseases caused by Neopestalotiopsis clavispora have been reported. To better understand the pathogenicity of N. clavispora, a solid fermentation strategy was employed to isolate and identify virulence factors afritoxinone B, afritoxinone A and oxysporone. The phytotoxic activities of these toxins were evaluated. Oxysporone exhibited high levels of phytotoxic activity after 72 h and the lesion area ranged from 21.5–84.3 mm2 after 9 days of treatment. The phytotoxic activities of the other two compounds were lower than that for oxysporone. The phytotoxic activity towards non-host organisms was also assessed for the three analyzed compounds; phytotoxic activity was observed in each case. Based on these results, we conclude that oxysporone is the main virulence factor in N. clavispora. We also suggest that each of the three compounds were non-host-specific toxins (NHST). To our knowledge, this is the first study to analyze phytotoxins produced by N. clavispora.

植物毒素广泛存在于植物病原体中。近年来，已经报道了由锁骨新孢子虫引起的许多疾病。为了更好地了解钉状猪笼草的致病性，采用固体发酵策略来分离和鉴定毒力因子Afritoxinone B，Afritoxinone A和羟孢霉素。评价了这些毒素的植物毒性活性。在治疗9天后，羟草胺在72 h后表现出高水平的植物毒性活性，病灶面积为21.5–84.3 mm2。其他两种化合物的植物毒性活性低于对羟孢菌素的毒性。还对三种分析的化合物对非宿主生物的植物毒性活性进行了评估。在每种情况下均观察到植物毒性活性。根据这些结果，我们得出结论，羟孢菌素是锁骨猪笼草的主要致病因子。。我们还建议这三种化合物中的每一种都是非宿主特异性毒素（NHST）。据我们所知，这是第一项分析猪笼草N. clavispora产生的植物毒素的研究。