A silver nanoparticle (AgNP)-based sandwich-type lateral flow immunoassay (LFIA) was evaluated for rapid detection of Staphylococcal enterotoxin B (SEB) in milk and honey. The role of trisodium citrate dihydrate (TSC) in the formation of Ag/TSC nanoparticles was established using UV–Vis spectroscopy. The association of silver with TSC in Ag/TSC nanoparticles was studied by the decrease in the intensity of anodic peak potential at 0.47 V and shift to 0.30 V in cyclic voltammetry (CV). The morphological, compositional and interaction studies of the AgNPs conjugated with the anti-SEB polyclonal antibody (Ag-sAb) was established using transmission electron microscopy (TEM) and X-ray photo electron spectroscopy (XPS) measurements. The visible detection limit and optical detection limit of the SEB test strip were 0.5 and 0.125 ppm, respectively, in SEB standard solution. This assay showed no cross-reaction with Staphylococcal enterotoxin A, Staphylococcal enterotoxin C or Salmonella typhi. Finally, the SEB test strip was effectively applied for the detection of SEB in spiked liquid milk and viscous honey, with optical detection limits of 0.25 and 0.5 ppm, respectively.

评估了一种基于银纳米颗粒（AgNP）的夹心型侧流免疫测定（LFIA），用于快速检测葡萄球菌肠毒素B（SEB）在牛奶和蜂蜜中。柠檬酸三钠二水合物（TSC）在Ag / TSC纳米颗粒形成中的作用是通过UV-Vis光谱法确定的。通过在循环伏安法（CV）中降低0.47 V处的阳极峰值电势强度并转移至0.30 V，研究了银与TSC在Ag / TSC纳米颗粒中的缔合。使用透射电子显微镜（TEM）和X射线光电子能谱（XPS）测量建立了与抗SEB多克隆抗体（Ag-sAb）偶联的AgNPs的形态，组成和相互作用研究。在SEB标准溶液中，SEB测试条的可见检出限和光学检出限分别为0.5和0.125 ppm。该测定显示与金黄色葡萄球菌肠毒素A，金黄色葡萄球菌肠毒素C或伤寒沙门氏菌没有交叉反应。