As part of a sustainable water resources management, the Lisbon municipality identified groundwater and treated wastewater use increase as two opportunities for better and sustainable water use, with natural safeguard for public health as a priority. In this context, the aim of our research was to assess the suitability of the human-associated marker gene Bacteroides HF183 and the cattle feces-associated CowM2, in routine water quality monitoring as indicators for water use and reuse, providing a tool to more accurately assess public health risks. To this intent, Real-Time quantitative PCR was used for detection of human-associated marker gene Bacteroides HF183 and the bovine-associated CowM2, in a total of 67 samples - groundwater and wastewater at three different treatment stages of a Waste Water Treatment Plant, in Lisbon. HF183 marker gene was detected in treated and untreated wastewater samples, with significant concentration reductions from untreated (6,07 E+07 copies/mL) to secondary treated effluent (1,86 E+05 copies/mL) and a further decrease in tertiary treatment (5,74 E+04 copies/mL). In groundwater samples, this marker was also detected in concentrations ranging from 2,63 E+02 copies/mL to 2,24 E+03 copies/mL. CowM2 marker gene on the other hand was only detected in wastewater samples, with concentrations ranging from 2,47 E+02 copies/mL to 1,17 E+04 copies/mL. Our research indicates that the use of Bacteroides spp. in association with traditional fecal indicator bacteria (FIB) is advantageous for water managing entities in urban settings, such as Lisbon, were drainage system failures may occur. An integrated approach thus provides crucial and more adequate information towards mitigation and correction measures when fecal contamination is detected in environmental waters.

作为可持续水资源管理的一部分，里斯本市确定地下水和废水处理量的增加是更好和可持续利用水的两个机会，其中优先考虑的是公共卫生的自然保障。在这种情况下，我们的研究目的是评估与人类相关的标记基因细菌拟杆菌HF183和与牛粪相关的CowM2在常规水质监测中作为水的使用和再利用的指标的适用性，为更准确地提供工具。评估公共健康风险。为此，实时定量PCR用于检测人类相关标记基因拟杆菌里斯本的HF183和与牛有关的CowM2，共67个样品-废水处理厂三个不同处理阶段的地下水和废水。在已处理和未处理的废水样品中检测到HF183标记基因，其浓度从未处理（6,07 E + 07拷贝/ mL）显着降低至二次处理的出水（1,86 E + 05拷贝/ mL），第三级进一步降低处理（5,74 E + 04拷贝/ mL）。在地下水样品中，该标志物的浓度也从2,63 E + 02拷贝/ mL到2,24 E + 03拷贝/ mL。另一方面，仅在废水样品中检测到CowM2标记基因，浓度范围为2,47 E + 02拷贝/ mL至1,17 E + 04拷贝/ mL。我们的研究表明，拟杆菌属的使用spp。与传统的粪便指示剂细菌（FIB）结合使用对于城市环境中的水管理实体（如里斯本）而言是有利的，因为排水系统可能会发生故障。因此，当在环境水域中检测到粪便污染时，综合方法可为缓解和纠正措施提供关键和更充分的信息。