The effect of electroporation parameters on the reversible permeabilization of cells in Thai basil leaves and, specifically, cells on the leaf surface was investigated, as electroporation of stomatal guard cells decreases drying times. Various combinations of PEF and MEF parameters were applied. The effect of these parameters on the electroporation of the leaf surface was assessed with microscopic observations and the electroporation of the bulk tissues was tested with electrical resistance measurements. With PEF and MEF, the electroporation of the epidermal cells increased with increasing treatment time. Compared to epidermal cells, guard cells required larger number of pulses to achieve homogeneous electroporation. Results showed that electroporation of cells on the leaf surface increased with the increase of voltage, pulse width and number of pulses. The electroporation of cells in the bulk tissues increased with increasing voltage and pulse width. Six specific combinations of parameters were found to electroporate the guard cells on the leaf surface while maintaining the viability of the leaves.

电穿孔参数对泰国罗勒叶中细胞可逆通透性的影响，特别是对叶片表面细胞的通透性的影响，因为气孔保卫细胞的电穿孔减少了干燥时间。应用了PEF和MEF参数的各种组合。通过显微镜观察评估这些参数对叶表面电穿孔的影响，并通过电阻测量测试大块组织的电穿孔。使用PEF和MEF，表皮细胞的电穿孔随治疗时间的增加而增加。与表皮细胞相比，保卫细胞需要更多数量的脉冲才能实现均匀的电穿孔。结果表明，叶片表面细胞的电穿孔随电压的升高而增加，脉冲宽度和脉冲数。大块组织中细胞的电穿孔随电压和脉冲宽度的增加而增加。发现六种特定的参数组合可以电穿孔叶片表面的保卫细胞，同时保持叶片的活力。