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Up-regulation of Squalene synthase in hairy root culture of Withania somnifera (L.) Dunal yields higher quantities of withanolides
Industrial Crops and Products ( IF 5.6 ) Pub Date : 2020-06-24 , DOI: 10.1016/j.indcrop.2020.112706
Ganeshan Sivanandhan , Natesan Selvaraj , Andy Ganapathi , Yong Pyo Lim

The roots of Indian ginseng, Withania somnifera (L.) Dunal (Solanaceae) are extensively used in ayurvedic and other traditional medicines. The plant produces a steroidal lactone, withanolide which has multiple health benefits. Squalene synthase plays a central role in regulating the withanolide biosynthetic pathway in W. somnifera. The present approach investigated the up-regulation of squalene synthase in withanolides biosynthesis pathway in hairy roots. The higher expression of squalene synthase was noticed in hairy root line Ws9 which exhibited withanolide A (2.82 mg/g DW), withanolide B (1.34 mg/g DW), withaferin A (1.83 mg/g DW) and withanone (1.97 mg/g DW) on the 40th day of culture with 1.08 to 1.25-times higher, respectively. Semi-quantitative and quantitative polymerase chain reaction confirmed the incorporation and expression of squalene synthase in cultured hairy roots.



中文翻译:

茄子(Withania somnifera(L.)Dunal)毛状根培养物中角鲨烯合酶的上调产生更多数量的醇类

印度人参,Withania somnifera(L.)Dunal(Solanaceae)的根被广泛用于阿育吠陀和其他传统药物。该植物产生甾醇类内酯withanolide,具有多种健康益处。角鲨烯合酶在调节W. somnifera中withanolide生物合成途径中起着核心作用。本方法研究了毛状根中withanolides生物合成途径中角鲨烯合酶上调。在有毛的根系Ws9中观察到角鲨烯合酶的较高表达,其表现出:甲酚A(2.82 mg / g DW),甲酚B(1.34 mg / g DW),甲递素A(1.83 mg / g DW)和甲酮(1.97 mg / g DW)40培养的天分别与更高1.08到1.25倍。半定量和定量聚合酶链反应证实了角鲨烯合酶在培养的毛根中的掺入和表达。

更新日期:2020-06-24
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