Synthetic polyaspartamides with various functional side groups including primary, secondary, tertiary amine or carboxyl groups were designed to explore the effect of chemical composition on polymer-mucin interactions. Since the molecular weight of the polymers and the degree of modification were identical for each derivative, the role of the functional groups could be evaluated. Chitosan was used as a control sample due to its strong interaction with mucin primarily through electrostatic forces. Mucoadhesive interactions of the polymers with the aqueous dispersion of commercially available porcine gastric mucin were probed on the colloid size scale using various methods including turbidimetric titration, dynamic light scattering and zeta potential measurements. Both the charge of the polymers and the type of amine groups had a pronounced effect on the interactions. The interactions were further analysed by partially screening them with either sodium chloride or urea. The results obtained allow us to classify these polymers in terms of in vitro mucoadhesive strength, which can be useful in the design of mucoadhesive formulations.

设计具有各种功能性侧基（包括伯，仲，叔胺或羧基）的合成聚天冬酰胺，以探索化学组成对聚合物-粘蛋白相互作用的影响。由于每种衍生物的聚合物分子量和修饰度均相同，因此可以评估官能团的作用。壳聚糖由于主要通过静电力与粘蛋白强烈相互作用，因此被用作对照样品。使用包括浊度滴定，动态光散射和ζ电势测量在内的各种方法，以胶体大小规模探测聚合物与可商购的猪胃粘蛋白的水分散体的粘膜粘附相互作用。聚合物的电荷和胺基的类型均对相互作用有显着影响。通过用氯化钠或尿素部分筛选它们来进一步分析相互作用。获得的结果使我们能够根据体外粘膜粘附强度对这些聚合物进行分类，这可用于设计粘膜粘附制剂。