The enzymatic mixture containing Novozyme Cellic CTec2 spiked with xylanase derived from Thermomyces lanuginosus (1000 U xylanase per g of substrate) resulted in enhanced saccharification of native, hydrothermally and autohydrolytically pretreated sugarcane bagasse when compared to the benchmark Cellic CTec2. Hydrothermally pretreated bagasse upon hydrolysis with the enzymatic mixture yielded considerably higher levels of sugars as compared to benchmark Cellic CTec2, resulting in saccharification efficiencies of 85.7, 77.8 and 78.9% at 10, 15 and 20% (weight per volume) substrate loadings, respectively. Autohydrolysed and native sugarcane bagasse samples had lower saccharification yields, although xylanase addition to the benchmark cocktail still was beneficial. Chemical compositional analysis and detailed morphological examination of the substrates after pretreatments as well as the enzymatic hydrolysis reactions employing confocal laser scanning microscopy (CLSM), field emission scanning electron microscopy (FESEM), X-Ray diffraction (XRD) and solid state nuclear magnetic resonance (ssNMR) provided fundamental insights into the structural changes during deconstruction and shed light on how such changes affected the biomass enzyme hydrolysis.

含Novozyme Cellic CTec2的酶混合物掺有源自嗜热霉菌的木聚糖酶（每克底物1000 U木聚糖酶）与基准Cellic CTec2相比，天然，水热和自水解预处理甘蔗渣的糖化作用增强。与基准Cellic CTec2相比，经酶混合物水解后进行水热预处理的蔗渣产生的糖水平明显更高，在10、15和20％（每体积重量）的底物负载量下，糖化效率分别为85.7％，77.8％和78.9％。尽管将木聚糖酶添加到基准鸡尾酒中仍然是有益的，但是自水解的和天然的甘蔗渣样品的糖化产率较低。