Water pollution as a result of contamination with dye-contaminating effluents is a severe issue for water reservoirs, which instigated the study of biodegradation of Reactive Red 195 and Reactive Blue dyes by E. coli and Bacillus sp. The effects of occupation time, solution pH, initial dyes concentrations, biomass loading, and temperature were investigated via batch-system experiments by using the Design of Experiment (DOE) for 2 levels and 5 factors response surface methodology (RSM). The operational conditions used for these factors were optimized using quadratic techniques by reducing the number of experiments. The results revealed that the two types of bacteria had a powerful effect on biodegradable dyes. The regression analysis revealed a good match of the experimental data to the second-order polynomial with a high coefficient of determination (R²). The optimum conditions achieved by E. coli were temperature (39.9 °C), initial concentration (99.6 mg L⁻¹), biomass loading (14.9 V_Biomass/V_Solution), incubation time (1 day), pH (7.23), while the optimum conditions achieved by Bacillus sp. were temperature (28.3 °C), initial concentration (98 mg L⁻¹), biomass loading (5.8 V_Biomass/V_Solution), incubation time (1 day), and pH (7.9) obtained from the desirability function.

染料污染废水污染导致的水污染对于水库来说是一个严重的问题，这引发了对大肠杆菌和芽孢杆菌对活性红195和活性蓝染料的生物降解的研究。sp。通过使用2级和5因子响应面方法（RSM）的实验设计（DOE），通过分批系统实验研究了占用时间，溶液pH值，初始染料浓度，生物量和温度的影响。通过减少实验次数，使用二次技术优化了用于这些因素的运行条件。结果表明，两种细菌对可生物降解的染料都有很强的作用。回归分析表明，实验数据与确定系数（R ²）高的二阶多项式匹配良好。大肠杆菌达到的最佳条件是温度（39.9°C），初始浓度（99.6 mg L ^-1）），生物量加载（14.9 V_生物质/ V_溶液），孵育时间（1天），pH（7.23），同时芽孢杆菌（Bacillus sp。）达到了最佳条件。从期望函数获得的温度是（28.3℃），初始浓度（98mg L ^-1），生物量负载（5.8V_生物量/ V_溶液），温育时间（1天）和pH（7.9）。