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Novel analytical approach to find distinctive odor compounds from garlic cloves infested by the potato-rot nematode Ditylenchus destructor using gas chromatography–olfactometry (GC–O) with heart-cut enrichment system
Journal of Plant Diseases and Protection ( IF 2.1 ) Pub Date : 2020-06-24 , DOI: 10.1007/s41348-020-00349-3
Misato Matsumoto , Daisuke Ueno , Rie Aoyama , Katsuhisa Sato , Yuki Koga , Taiki Higuchi , Hideaki Matsumoto , Kou Nishimuta , Shunsuke Haraguchi , Hideki Miyamoto , Tomokazu Haraguchi , Toyoshi Yoshiga

The potato-rot nematode Ditylenchus destructor (Tylenchida: Anguinidae) causes severe damage to garlic production in Japan. Nematode-contaminated garlic seed cloves are often used for planting, because nematode-infested cloves are difficult to discriminate from healthy ones. To be able to find nematode-infested garlic in a nondestructive way, here this study introduced a novel analytical system of GC–O and GC–MS with heart-cut enrichment technique. The objective of this research was to find an analytical approach to identify the existence of subjectively perceptible odors which derive from the garlic infested with the nematode and to evaluate the effectiveness of those novel analytical systems. Ten odor activities associated with nematode-infested garlic were detected by the system, and “allyl methyl sulfide” and “2-methyl-1-butanol” were identified as major odor compounds (1-propanethiol was candidate). This result suggests that the novel analytical technique introduced in this study delivered sufficient data to identify the odor compounds from infested garlics. Two distinctive odor compounds might be used as indicators to detect nematode-infested garlic cloves with nondestructive way.

中文翻译:

新颖的分析方法,通过使用心切富集系统的气相色谱-嗅觉法(GC-O)从被马铃薯腐烂线虫Ditylenchus破坏物侵染的蒜瓣中发现独特的气味化合物

马铃薯腐烂线虫Ditylenchus破坏物(Tylenchida:Anguinidae)对日本的大蒜生产造成严重损害。受线虫污染的大蒜种子丁香通常用于种植,因为难以将线虫感染的丁香丁与健康的丁香区别开。为了能够以一种非破坏性的方式找到线虫感染的大蒜,本研究引入了一种采用心切富集技术的新型GC-O和GC-MS分析系统。这项研究的目的是找到一种分析方法,以识别存在于线虫中的大蒜所产生的主观可闻气味,并评估这些新颖分析系统的有效性。该系统检测到十种与线虫感染的大蒜有关的气味活动,和“烯丙基甲基硫醚”和“ 2-甲基-1-丁醇”被确定为主要的气味化合物(1-丙硫醇是候选化合物)。该结果表明,本研究中引入的新颖分析技术提供了足够的数据,可从受感染的大蒜中识别出气味化合物。可以使用两种独特的气味化合物作为指示剂,以非破坏性的方式检测线虫感染的大蒜。
更新日期:2020-06-24
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