Pediocin PA-1 is a bacteriocin that shows strongly anti-microbial activity against some Gram-positive pathogens such as Listeria monocytogenes, Staphylococcus aureus, and Enterococcus faecalis. With the broad inhibitory spectrum as well as high-temperature stability, pediocin has a potential application in the food preservation and pharmaceutical industry. Pediocin has been studied to express in many heterologous expression systems such as Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris as a free peptide. Here we showed in this study a new strategy by using yeast surface display system to produce the anchored pediocin PA-1 on the cell surface of Saccharomyces cerevisiae, which could be used directly as a pediocin resource. We had successfully constructed a recombinant S. cerevisiae W303 strain that could express pediocin PA-1 on the cell surface. The pediocin-expressing yeast could inhibit the growth of Shigella boydii and Shigella flexneri, which have never been reported before for pediocin activity. Besides, the pediocin expression level of the recombinant S. cerevisiae strain was also evaluated in three different media: synthetic defined (SD), basic medium (BM), and fermentation medium (FM). BM medium was shown to give the highest production yield of the recombinant yeast (4.75 ± 0.75 g dry cell weight per 1 L of culture) with the ratio number of the pediocin-expressing cells of 93.46 ± 2.45%. Taken together, the results clearly showed that pediocin can be displayed on yeast cell surface as anchored protein. The application of yeast cell surface system enables a new door of pediocin application on either food or feed industries.

Graphical abstract

Pediocin PA-1是一种细菌素，对某些革兰氏阳性病原体（如单核细胞增生性李斯特菌，金黄色葡萄球菌和粪肠球菌）具有很强的抗微生物活性。pediocin具有广泛的抑制谱以及高温稳定性，因此在食品保存和制药行业中具有潜在的应用前景。已经研究了Pediocin在许多异源表达系统中以游离肽的形式表达，例如大肠杆菌，酿酒酵母和巴斯德毕赤酵母。在这里，我们在这项研究中展示了一种通过使用酵母表面展示系统在锚定细胞表面产生锚定的pediocin PA-1的新策略。酿酒酵母（Saccharomyces cerevisiae），可以直接用作pediocin资源。我们已经成功地构建了重组小号。可以在细胞表面表达pediocin PA-1的酿酒酵母W303菌株。表达pediocin的酵母可以抑制博伊氏志贺氏菌和弗氏志贺氏菌的生长，这以前没有关于pediocin活性的报道。此外，重组S的花青素表达水平。酿酒酵母还用三种不同的培养基对菌株进行了评估：合成培养基（SD），基本培养基（BM）和发酵培养基（FM）。BM培养基显示出重组酵母的最高产量（每1L培养物4.75±0.75g干细胞重量），表达pediocin的细胞的比例为93.46±2.45％。两者合计，结果清楚地表明，pediocin可作为锚定蛋白在酵母细胞表面展示。酵母细胞表面系统的应用为食品或饲料工业中的pediocin应用开辟了新的大门。

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