Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (FOC) is an important soil-borne disease of cucumber in several parts of the world. In this study, we employed amplified fragment length polymorphic (AFLP) markers along with bulked segregant analysis (BSA), in order to identify structural markers linked to the Foc gene that governs resistance to FOC races 1, 2 and 3 in the resistant cv. SMR-18. From the four AFLP markers originally identified at the BSA level, only one (E12M50_134 + T) maintained a linkage in coupling phase against the F₂ mapping population. AFLP-mediated sequencing was employed in order to ensure the identification and sequencing of the truly linked AFLP fragment. This AFLP marker mapped at a distance of 6.0 cM from the Foc resistance locus and was successfully converted into two sequence-characterized amplified region (SCAR) co-dominant markers. SCAR marker SCE12M50_B produced a single fragment linked to Foc and behaved as a dominant marker, while SCE12M50_A amplified one strong fragment linked to Foc in parallel with a second faint fragment. The SCE12M50_B fragment physically maps on chromosome 2 and lies 1.64 Mb (7,0 cM) away from the microsatellite marker SSR03084, which is closely linked to Ccu locus that controls resistance to scab, caused by Cladosporium cucumerinum in cv. SMR-18.

枯萎病，引起枯萎病菌F。sp。黄瓜（FOC）是世界上许多地方的一种重要的黄瓜土传病害。在这项研究中，我们采用了扩增的片段长度多态性（AFLP）标记以及大量的分离物分析（BSA），以鉴定与Foc基因相关的结构标记，该基因控制抗性cv中对FOC第1、2和3种的抗性。SMR-18。在最初在BSA级别上识别出的四个AFLP标记中，只有一个（E12M50 _{134 + T}）在偶联阶段与F ₂保持连锁绘制人口图。采用AFLP介导的测序以确保鉴定和测序真正连接的AFLP片段。该AFLP标记位于距Foc抗性基因座6.0 cM的位置，并成功地转换为两个序列表征的扩增区（SCAR）共显性标记。SCAR标记SCE12M50 _B产生一个与Foc连接的单个片段，并作为显性标记，而SCE12M50 _A扩增了一个与Foc连接的强片段，与另一个微弱的片段平行。SCE12M50 _B片段物理定位在2号染色体上，与微卫星标记SSR03084距离1.64 Mb（7.0 cM），与控制cuc对结controls的抵抗力的ccu基因座，该病因是黄瓜中的隐孢子虫。SMR-18。