Despite the wide choice of commercial heart valve prostheses, cryopreserved semilunar allograft heart valves (C-AHV) are required, and successfully transplanted in selected groups of patients. The expiration limit (EL) criteria have not been defined yet. Most Tissue Establishments (TE) use the EL of 5 years. From physiological, functional, and surgical point of view, the morphology and mechanical properties of aortic and pulmonary roots represent basic features limiting the EL of C-AHV. The aim of this work was to review methods of AHV tissue structural analysis and mechanical testing from the perspective of suitability for EL validation studies. Microscopic structure analysis of great arterial wall and semilunar leaflets tissue should clearly demonstrate cells as well as the extracellular matrix components by highly reproducible and specific histological staining procedures. Quantitative morphometry using stereological grids has proved to be effective, as the exact statistics was feasible. From mechanical testing methods, tensile test was the most suitable. Young’s moduli of elasticity, ultimate stress and strain were shown to represent most important AHV tissue mechanical characteristics, suitable for exact statistical analysis. C-AHV are prepared by many different protocols, so as each TE has to work out own EL for C-AHV.

尽管有多种商业心脏瓣膜假体可供选择，但仍需要冷冻保存的半月同种异体心脏瓣膜 (C-AHV)，并成功移植到选定的患者组中。到期限制 (EL) 标准尚未定义。大多数组织机构 (TE) 使用 5 年的 EL。从生理、功能和手术的角度来看，主动脉和肺根的形态和机械特性代表了限制 C-AHV EL 的基本特征。这项工作的目的是从 EL 验证研究的适用性角度审查 AHV 组织结构分析和机械测试的方法。大动脉壁和半月叶组织的显微结构分析应通过高度可重复和特定的组织学染色程序清楚地显示细胞以及细胞外基质成分。使用立体网格的定量形态测量已被证明是有效的，因为精确的统计是可行的。从力学测试方法来看，拉伸测试是最合适的。杨氏弹性模量、极限应力和应变显示出最重要的 AHV 组织机械特性，适用于精确的统计分析。C-AHV 由许多不同的协议准备，因此每个 TE 必须为 C-AHV 制定自己的 EL。从力学测试方法来看，拉伸测试是最合适的。杨氏弹性模量、极限应力和应变显示出最重要的 AHV 组织机械特性，适用于精确的统计分析。C-AHV 由许多不同的协议准备，因此每个 TE 必须为 C-AHV 制定自己的 EL。从力学测试方法来看，拉伸测试是最合适的。杨氏弹性模量、极限应力和应变显示出最重要的 AHV 组织机械特性，适用于精确的统计分析。C-AHV 由许多不同的协议准备，因此每个 TE 必须为 C-AHV 制定自己的 EL。