The combination of marine origin biopolymers for tissue engineering (TE) applications is of high interest, due to their similarities with the proteins and polysaccharides present in the extracellular matrix of different human tissues. This manuscript reports on innovative collagen-chitosan-fucoidan cryogels formed by the simultaneous blending of these three marine polymers in a chemical-free crosslinking approach. The physicochemical characterization of marine biopolymers comprised FTIR, amino acid analysis, circular dichroism and SDS-PAGE, and suggested that the jellyfish collagen used in the cryogels was not denatured (preserved the triple helical structure) and had similarities with type II collagen. The chitosan presented a high deacetylation degree (90.1%) that can strongly influence the polymer physicochemical properties and biomaterial formation. By its turn, rheology, and SEM studies confirmed that these novel cryogels present interesting properties for TE purposes, such...

中文翻译：

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海洋胶原蛋白-壳聚糖-岩藻依聚糖冷冻凝胶作为载有细胞的生物复合材料，可用于组织工程。

用于组织工程 (TE) 应用的海洋生物聚合物的组合引起了人们的高度关注，因为它们与不同人体组织的细胞外基质中存在的蛋白质和多糖具有相似性。这份手稿报告了由这三种海洋聚合物在无化学交联方法中同时混合形成的创新胶原蛋白-壳聚糖-岩藻依聚糖冷冻凝胶。海洋生物聚合物的物理化学表征包括 FTIR、氨基酸分析、圆二色性和 SDS-PAGE，表明冷冻凝胶中使用的水母胶原蛋白未变性（保留三螺旋结构）并且与 II 型胶原蛋白有相似之处。壳聚糖呈现高脱乙酰度（90. 1%) 可以强烈影响聚合物的物理化学性质和生物材料的形成。反过来，流变学和 SEM 研究证实，这些新型冷冻凝胶具有用于 TE 目的的有趣特性，例如……