Abstract Citrus plants have been extremely affected by Huanglongbing (HLB) worldwide, causing economic losses. HLB disease causes disorders in citrus plants, leading to callose deposition in the phloem vessel sieve plates. Callose is synthesized by callose synthases, which are encoded by 12 genes (calS1– calS12)in Arabidopsis thaliana. We evaluated the expression of eight callose synthase genes from Citrus in hybrids between Citrus sunki and Poncirus trifoliata infected with HLB. The objective of this work was to identify possible tolerance loci combining the expression quantitative trait loci (eQTL) of different callose synthases and genetic Single-Nucleotide Polymorphism (SNP) maps of C. sunki and P. trifoliata. The expression data from all CscalS ranged widely among the hybrids. Furthermore, the data allowed the detection of 18 eQTL in the C. sunki map and 34 eQTL in the P. trifoliata map. In both maps, some eQTL for different CscalS were overlapped; thus, a single region could be associated with the regulation of more than one CscalS. The regions identified in this work can be interesting targets for future studies of Citrus breeding programs to manipulate callose synthesis during HLB infection.

中文翻译：

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用细菌 Candidatus Liberibacter asiaticus 攻击的柑橘属间杂种中胼胝合酶的表达定量性状基因座 (eQTL) 作图

摘要 柑橘植物在世界范围内受到黄龙病（HLB）的严重影响，造成经济损失。HLB 病会导致柑橘类植物紊乱，导致韧皮部血管筛板中的胼胝质沉积。胼胝质由胼胝质合酶合成，由拟南芥中的 12 个基因 (calS1–calS12) 编码。我们评估了来自柑橘的八个胼胝质合酶基因在感染 HLB 的 Citrus sunki 和 Poncirus trifoliata 之间的杂种中的表达。这项工作的目的是结合不同胼胝合酶的表达数量性状基因座 (eQTL) 和 C. sunki 和 P. trifoliata 的遗传单核苷酸多态性 (SNP) 图，确定可能的耐受基因座。来自所有 CscalS 的表达数据在杂交种中范围广泛。此外，该数据允许在 C. 中检测到 18 个 eQTL。sunki 图和 P. trifoliata 图中的 34 eQTL。在这两个图谱中，不同 CscalS 的一些 eQTL 是重叠的；因此，单个区域可能与多个 CscalS 的调节相关。在这项工作中确定的区域可能是未来研究柑橘育种计划以在 HLB 感染期间操纵胼胝质合成的有趣目标。