BACKGROUND:Fourier-transform infrared spectroscopy (FTIR) is one of the standard methods to analyze ultra-high molecular weight polyethylene (UHMWPE) in orthopedic implants. For retrieved components, lipid extraction using an organic solvent prior to the measurement is necessary to eliminate the influence of lipids absorbed in vivo. However, its influence on the measurement has not been substantially investigated. OBJECTIVE:To investigate the influence of lipid extraction on the FTIR analysis of UHMWPE and to develop a novel method to obtain reliable results without inconvenient lipid extraction. METHODS:FTIR analysis was repeatedly performed on UHMWPE specimens from retrieved components before and after lipid extraction under various conditions. A method to calculate the extent of influence of the absorbed lipids from the FTIR spectra was developed using a peak separation technique. RESULTS:An elevated temperature was necessary for lipid extraction; however, it had the potential to influence the results if the conditions were not properly controlled. The results obtained using the peak separation technique coincided with those obtained after lipid extraction. CONCLUSION:The use of the peak separation technique enables the efficient acquisition of reliable results without the need for lipid extraction.

中文翻译：

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一种使用傅里叶变换红外光谱法消除吸收的脂质对超高分子量聚乙烯表征影响的新方法。

背景：傅立叶变换红外光谱（FTIR）是分析整形外科植入物中超高分子量聚乙烯（UHMWPE）的标准方法之一。对于回收的成分，必须在测量之前使用有机溶剂提取脂质，以消除体内吸收的脂质的影响。但是，其对测量的影响尚未得到实质性研究。目的：研究脂质提取对UHMWPE FTIR分析的影响，并开发出一种新的方法来获得可靠的结果，而不会造成脂质提取的不便。方法：在各种条件下，在提取脂质之前和之后，对回收的UHMWPE标本重复进行FTIR分析。开发了一种使用峰分离技术从FTIR光谱计算吸收的脂质影响程度的方法。结果：脂质提取需要升高温度。但是，如果条件得不到适当控制，它有可能影响结果。使用峰分离技术获得的结果与脂质提取后获得的结果一致。结论：使用峰分离技术可以有效地获得可靠的结果，而无需进行脂质提取。使用峰分离技术获得的结果与脂质提取后获得的结果一致。结论：使用峰分离技术可以有效地获得可靠的结果，而无需进行脂质提取。使用峰分离技术获得的结果与脂质提取后获得的结果一致。结论：使用峰分离技术可以有效地获得可靠的结果，而无需进行脂质提取。