Over the past decade, genetically encoded fluorescent biosensors that report metabolic changes have become valuable tools for understanding brain metabolism. These sensors have been targeted to specific brain regions and cell types in different organisms to track multiple metabolic processes at single cell (and subcellular) resolution. Here, we review genetically encoded biosensors used to study metabolism in the brain. We particularly focus on the principles needed to use these sensors quantitatively while avoiding false inferences from variations in sensor fluorescence that arise from differences in expression level or environmental influences such as pH or temperature.

中文翻译：

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用于神经元代谢的荧光生物传感器和定量挑战。

在过去的十年中，报告代谢变化的基因编码荧光生物传感器已成为了解大脑代谢的宝贵工具。这些传感器已针对不同生物体的特定大脑区域和细胞类型，以单细胞（和亚细胞）分辨率跟踪多个代谢过程。在这里，我们回顾了用于研究大脑新陈代谢的基因编码生物传感器。我们特别关注定量使用这些传感器所需的原则，同时避免由于表达水平或环境影响（如 pH 值或温度）的差异而引起的传感器荧光变化的错误推断。