Abstract The objective of this study was to evaluate the level of genomic instability in patients with celiac disease and to establish a relationship between inflammation, oxidative stress, and DNA damage in these patients. Myeloperoxidase (MPO) activity, adenosine deaminase, nitric oxide (NOx), thiobarbituric acid, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and DNA damage were evaluated in peripheral blood samples from 47 celiac disease patients and 31 controls. Patients with celiac disease presented higher levels of DNA damage in comparison to controls (p=0.023). This difference was also observed for markers of oxidative stress, such as CAT (p=0.011) and SOD (p=0.013), and inflammatory markers such as MPO (p < 0.001) and NOx (p=0.009). Positive correlations were found between DNA damage levels and the values of CAT (r=0.405; p=0.009) and SOD (r=0.516; p < 0.001). Positive correlations were also found between GPx and NOx (r=0.349; p=0.030) and MPO and NOx (r=0.239; p=0.039). CAT and NOx showed a negative correlation (r= −0.315; p=0.042). In conclusion, intestinal inflammation can have systemic effects, causing an imbalance between oxidant and antioxidant markers, which may promote increased levels of DNA damage.

中文翻译：

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乳糜泻患儿的 DNA 损伤、氧化应激和炎症

摘要 本研究的目的是评估乳糜泻患者的基因组不稳定性水平，并确定这些患者的炎症、氧化应激和 DNA 损伤之间的关系。在来自 47 名乳糜泻患者的外周血样本中评估了髓过氧化物酶 (MPO) 活性、腺苷脱氨酶、一氧化氮 (NOx)、硫代巴比妥酸、过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GPx) 和 DNA 损伤。 31个控件。与对照组相比，乳糜泻患者的 DNA 损伤水平更高（p=0.023）。对于氧化应激标记物（例如 CAT (p=0.011) 和 SOD (p=0.013)）以及炎症标记物（例如 MPO (p < 0.001) 和 NOx (p=0.009)）也观察到了这种差异。在 DNA 损伤水平与 CAT (r=0.405; p=0.009) 和 SOD (r=0.516; p < 0.001) 值之间发现正相关。在 GPx 和 NOx（r=0.349；p=0.030）和 MPO 和 NOx（r=0.239；p=0.039）之间也发现了正相关。CAT 和 NOx 呈负相关（r= -0.315；p=0.042）。总之，肠道炎症会产生全身性影响，导致氧化剂和抗氧化标记物之间的不平衡，这可能会促进 DNA 损伤水平的增加。